Aberrant microRNAs Expression in CD133+/CD326+ Human Lung Adenocarcinoma Initiating Cells from A549

被引：47

作者：

Lin, Sheng ^{[1
]}

Sun, Jian-guo ^{[1
]}

Wu, Jing-bo

Long, Hai-xia ^{[1
]}

Zhu, Cong-hui ^{[1
]}

Xiang, Tong ^{[1
]}

Ma, Hu ^{[1
]}

Zhao, Zhong-quan ^{[1
]}

Yao, Quan ^{[1
]}

Zhang, An-mei ^{[1
]}

Zhu, Bo ^{[1
]}

Chen, Zheng-tang ^{[1
]}

机构：

[1] Third Mil Med Univ, Xinqiao Hosp, Inst Canc, Chongqing 400037, Peoples R China

来源：

MOLECULES AND CELLS | 2012年 / 33卷 / 03期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

A549; microarray; microRNA; paclitaxel; tumor initiating cells; CANCER STEM-CELLS; DOWN-REGULATION; SELF-RENEWAL; IDENTIFICATION; CD133(+); GROWTH; PROLIFERATION; LIVER;

D O I：

10.1007/s10059-012-2252-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Increasing evidence demonstrates that miRNAs are involved in the dysregulation of tumor initiating cells (TICs) in various tumors. Due to a lack of definitive markers, cell sorting is not an ideal separation method for lung adenocarcinoma initiating cells. In this study, we combined paclitaxel with serum-free medium cultivation (inverse-induction) to enrich TICs from A549 cells, marked by CD133/CD326, defined features of stemness. We next investigated aberrant microRNAs in this subpopulation compared to normal cells with miRNA microarray and found that 50 miRNAs exhibited a greater than 2-fold change in expression. As further validation, 10 miRNAs were chosen to perform quantitative RT-PCR on the A549 cell line and primary samples. The results suggest that aberrant expression of miRNAs such as miR-29ab, miR-183, miR-175p and miR-127-3P may play an important role in regulating the bio-behavior of TICs.

引用

页码：277 / 283

页数：7

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