Aberrant microRNAs Expression in CD133+/CD326+ Human Lung Adenocarcinoma Initiating Cells from A549

被引：46

作者：

Lin, Sheng ^{[1
]}

Sun, Jian-guo ^{[1
]}

Wu, Jing-bo

Long, Hai-xia ^{[1
]}

Zhu, Cong-hui ^{[1
]}

Xiang, Tong ^{[1
]}

Ma, Hu ^{[1
]}

Zhao, Zhong-quan ^{[1
]}

Yao, Quan ^{[1
]}

Zhang, An-mei ^{[1
]}

Zhu, Bo ^{[1
]}

Chen, Zheng-tang ^{[1
]}

机构：

[1] Third Mil Med Univ, Xinqiao Hosp, Inst Canc, Chongqing 400037, Peoples R China

来源：

MOLECULES AND CELLS | 2012年 / 33卷 / 03期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

A549; microarray; microRNA; paclitaxel; tumor initiating cells; CANCER STEM-CELLS; DOWN-REGULATION; SELF-RENEWAL; IDENTIFICATION; CD133(+); GROWTH; PROLIFERATION; LIVER;

D O I：

10.1007/s10059-012-2252-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Increasing evidence demonstrates that miRNAs are involved in the dysregulation of tumor initiating cells (TICs) in various tumors. Due to a lack of definitive markers, cell sorting is not an ideal separation method for lung adenocarcinoma initiating cells. In this study, we combined paclitaxel with serum-free medium cultivation (inverse-induction) to enrich TICs from A549 cells, marked by CD133/CD326, defined features of stemness. We next investigated aberrant microRNAs in this subpopulation compared to normal cells with miRNA microarray and found that 50 miRNAs exhibited a greater than 2-fold change in expression. As further validation, 10 miRNAs were chosen to perform quantitative RT-PCR on the A549 cell line and primary samples. The results suggest that aberrant expression of miRNAs such as miR-29ab, miR-183, miR-175p and miR-127-3P may play an important role in regulating the bio-behavior of TICs.

引用

页码：277 / 283

页数：7

共 45 条

[1] Prospective identification of tumorigenic breast cancer cells [J].

Al-Hajj, M ;

Wicha, MS ;

Benito-Hernandez, A ;

Morrison, SJ ;

Clarke, MF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (07) :3983-3988

[2] Highly tumorigenic lung cancer CD133+ cells display stem-like features and are spared by cisplatin treatment [J].

Bertolini, Giulia ;

Roz, Luca ;

Perego, Paola ;

Tortoreto, Monica ;

Fontanella, Enrico ;

Gatti, Laura ;

Pratesi, Graziella ;

Fabbri, Alessandra ;

Andriani, Francesca ;

Tinelli, Stella ;

Roz, Elena ;

Caserini, Roberto ;

Lo Vullo, Salvatore ;

Camerini, Tiziana ;

Mariani, Luigi ;

Delia, Domenico ;

Calabro, Elisa ;

Pastorino, Ugo ;

Sozzi, Gabriella .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (38) :16281-16286

[3] Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell [J].

Bonnet, D ;

Dick, JE .

NATURE MEDICINE, 1997, 3 (07) :730-737

[4] Positive correlations of Oct-4 and Nanog in oral cancer stem-like cells and high-grade oral squamous cell carcinoma [J].

Chiou, Shih-Hwa ;

Yu, Cheng-Chia ;

Huang, Chi-Yang ;

Lin, Shu-Chun ;

Liu, Chung-Ji ;

Tsai, Tung-Hu ;

Chou, Shiu-Huey ;

Chien, Chian-Shiu ;

Ku, Hung-Hai ;

Lo, Jeng-Fan .

CLINICAL CANCER RESEARCH, 2008, 14 (13) :4085-4095

[5] The miR-17-5p microRNA is a key regulator of the G1/S phase cell cycle transition [J].

Cloonan, Nicole ;

Brown, Mellissa K. ;

Steptoe, Anita L. ;

Wani, Shivangi ;

Chan, Wei Ling ;

Forrest, Alistair Rr ;

Kolle, Gabriel ;

Gabrielli, Brian ;

Grimmond, Sean M. .

GENOME BIOLOGY, 2008, 9 (08)

[6] Prostate cancer stem cells [J].

Collins, Anne T. ;

Maitland, Norman J. .

EUROPEAN JOURNAL OF CANCER, 2006, 42 (09) :1213-1218

[7] MicroRNA Regulation of Cancer Stem Cells and Therapeutic Implications [J].

DeSano, Jeffrey T. ;

Xu, Liang .

AAPS JOURNAL, 2009, 11 (04) :682-692

[8] Identification and expansion of the tumorigenic lung cancer stem cell population [J].

Eramo, A. ;

Lotti, F. ;

Sette, G. ;

Pilozzi, E. ;

Biffoni, M. ;

Di Virgilio, A. ;

Conticello, C. ;

Ruco, L. ;

Peschle, C. ;

De Maria, R. .

CELL DEATH AND DIFFERENTIATION, 2008, 15 (03) :504-514

[9] MicroRNA-199b-5p Impairs Cancer Stem Cells through Negative Regulation of HES1 in Medulloblastoma [J].

Garzia, Livia ;

Andolfo, Immacolata ;

Cusanelli, Emilio ;

Marino, Natascia ;

Petrosino, Giuseppe ;

De Martino, Daniela ;

Esposito, Veronica ;

Galeone, Aldo ;

Navas, Luigi ;

Esposito, Silvia ;

Gargiulo, Sara ;

Fattet, Sarah ;

Donofrio, Vittoria ;

Cinalli, Giuseppe ;

Brunetti, Arturo ;

Del Vecchio, Luigi ;

Northcott, Paul A. ;

Delattre, Olivier ;

Taylor, Michael D. ;

Iolascon, Achille ;

Zollo, Massimo .

PLOS ONE, 2009, 4 (03)

[10] Targeting of the Bmi-1 Oncogene/Stem Cell Renewal Factor by MicroRNA-128 Inhibits Glioma Proliferation and Self-Renewal [J].

Godlewski, Jakub ;

Nowicki, Michal O. ;

Bronisz, Agnieszka ;

Williams, Shante ;

Otsuki, Akihiro ;

Nuovo, Gerard ;

RayChaudhury, Abhik ;

Newton, Herbert B. ;

Chiocca, E. Antonio ;

Lawler, Sean .

CANCER RESEARCH, 2008, 68 (22) :9125-9130

← 1 2 3 4 5 →