Recovery of immune activity by administration of polysaccharides ofToona sinensisand its characterization of major component

The polysaccharides were acquired from the seeds ofT. sinensisby hot water extraction, ethanol precipitation, decoloration with macroporous 900 resin, and deproteinization with sevag reagent, which possessed significantly immunomodulatory activity on the levels of IgA, IgG, and IgM, the relative indices of thymus and spleen, and the phagocytosis index of mice invivo. T. sinensisseeds polysaccharides (TSP) were separated and purified using DEAE-52 cellulose and Sephacryl S-300 column chromatography to obtain TSP-3a characterized of molecular weight, monosaccharides composition, and other preliminary structural features. TSP-3a (758.6 kDa) mainly contained Man, GalN, GlcA, Rha, Glc, Gal, Ara, and Fuc in a molar ratio of 0.26:0.24:0.19:1.80:0.45:12.56:1.09:0.21. FT-IR analysis suggested TSP-3a was an acid polysaccharide and the presence of beta configuration within the TSP-3a was discovered. Combining(1)H NMR,C-13 NMR, and comparison with those reported in the literature, the backbone of TSP-3a might contain -> 6)-alpha-D-Glc-(1 ->, -> 3)-beta-D-GlcA-(1 ->, -> 6)-alpha-D-Gal-(1 ->,alpha-D-Glc-(1 ->, and -> 6)-beta-D-Gal-(1 ->.