Phosphorylated SATB1 is associated with the progression and prognosis of glioma

被引：37

作者：

Han, S. ^{[1
]}

Xia, J. ^{[1
]}

Qin, X. ^{[1
]}

Han, S. ^{[1
]}

Wu, A. ^{[1
]}

机构：

[1] China Med Univ, Hosp 1, Dept Neurosurg, Shenyang 110001, Peoples R China

来源：

CELL DEATH & DISEASE | 2013年 / 4卷

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

glioblastoma multiforme; special AT-rich sequence-binding protein 1; phosphorylation; prognosis; GENE-EXPRESSION; GASTRIC-CANCER; STEM-CELL; GROWTH; TRANSCRIPTION; ORGANIZER; SURVIVAL; INVASION; REVEALS; TISSUES;

D O I：

10.1038/cddis.2013.433

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Special AT-rich sequence-binding protein 1 (SATB1) is a global chromatin organizer and gene regulator, and high expression of SATB1 is associated with progression and poor prognosis in several malignancies. Here, we examine the expression pattern of SATB1 in glioma. Microarray analysis of 127 clinical samples showed that SATB1 mRNA was expressed at lower levels in highly malignant glioblastoma multiforme (GBM) than in low-grade glioma and normal brain tissue. This result was further confirmed by real-time RT-PCR in the clinical samples, three GBM cell lines, primary SU3 glioma cells and tumor cells harvested by laser-capture microdissection. Consistent with the mRNA levels, SATB1 protein expression was downregulated in high-grade glioma, as shown by western blotting. However, phospho-SATB1 levels showed an opposite pattern, with a significant increase in these tumors. Immunohistochemical analysis of phospho-SATB1 expression in tissue microarrays with tumors from 122 glioma cases showed that phospho-SATB1 expression was significantly associated with high histological grade and poor survival by Kaplan-Meier analysis. In vitro transfection analysis showed that phospho-SATB1 DNA binding has a key role in regulating the proliferation and invasion of glioma cells. The effect of SATB1 in glioma cell is mainly histone deacetylase (HDAC1)-dependent. We conclude that phospho-SATB1, but not SATB1 mRNA expression, is associated with the progression and prognosis of glioma. By interaction with HDAC1, phospho-SATB1 contributes to the invasive and proliferative phenotype of GBM cells.

引用

页码：e901 / e901

页数：10

共 40 条

[21]

Notani D, 2010, METHODS MOL BIOL, V647, P317, DOI 10.1007/978-1-60761-738-9_20

[22] The mRNA expression of SATB1 and SATB2 in human breast cancer [J].

Patani, Neill ;

Jiang, Wen ;

Mansel, Robert ;

Newbold, Robert ;

Mokbel, Kefah .

CANCER CELL INTERNATIONAL, 2009, 9

[23] Phosphorylation of SATB1, a global gene regulator, acts as a molecular switch regulating its transcriptional activity in vivo [J].

Pavan, KP ;

Purbey, PK ;

Sinha, CK ;

Notani, D ;

Limaye, A ;

Jayani, RS ;

Galande, S .

MOLECULAR CELL, 2006, 22 (02) :231-243

[24] Disrupting the PIKE-A/Akt interaction inhibits glioblastoma cell survival, migration, invasion and colony formation [J].

Qi, Q. ;

He, K. ;

Liu, X. ;

Pham, C. ;

Meyerkord, C. ;

Fu, H. ;

Ye, K. .

ONCOGENE, 2013, 32 (08) :1030-1040

[25] Wnt activation promotes neuronal differentiation of Glioblastoma [J].

Rampazzo, E. ;

Persano, L. ;

Pistollato, F. ;

Moro, E. ;

Frasson, C. ;

Porazzi, P. ;

Della Puppa, A. ;

Bresolin, S. ;

Battilana, G. ;

Indraccolo, S. ;

Te Kronnie, G. ;

Argenton, F. ;

Tiso, N. ;

Basso, G. .

CELL DEATH & DISEASE, 2013, 4 :e500-e500

[26] Astrocytes Enhance the Invasion Potential of Glioblastoma Stem-Like Cells [J].

Rath, Barbara H. ;

Fair, Joshlean M. ;

Jamal, Muhammad ;

Camphausen, Kevin ;

Tofilon, Philip J. .

PLOS ONE, 2013, 8 (01)

[27] Connective Tissue Growth Factor (CTGF/CCN2) Is Negatively Regulated during Neuron-Glioblastoma Interaction [J].

Romao, Luciana F. ;

Mendes, Fabio A. ;

Feitosa, Natalia M. ;

Faria, Jane Cristina O. ;

Coelho-Aguiar, Juliana M. ;

de Souza, Jorge Marcondes ;

Moura Neto, Vivaldo ;

Abreu, Jose Garcia .

PLOS ONE, 2013, 8 (01)

[28] Leptin Reduces the Expression and Increases the Phosphorylation of the Negative Regulators of GLUT4 Traffic TBC1D1 and TBC1D4 in Muscle of ob/ob Mice [J].

Sainz, Neira ;

Rodriguez, Amaia ;

Catalan, Victoria ;

Becerril, Sara ;

Ramirez, Beatriz ;

Lancha, Andoni ;

Burgos-Ramos, Emma ;

Gomez-Ambrosi, Javier ;

Fruehbeck, Gema .

PLOS ONE, 2012, 7 (01)

[29] Radiotherapy plus concomitant and adjuvant temozolomide for glioblastoma [J].

Stupp, R ;

Mason, WP ;

van den Bent, MJ ;

Weller, M ;

Fisher, B ;

Taphoorn, MJB ;

Belanger, K ;

Brandes, AA ;

Marosi, C ;

Bogdahn, U ;

Curschmann, J ;

Janzer, RC ;

Ludwin, SK ;

Gorlia, T ;

Allgeier, A ;

Lacombe, D ;

Cairncross, JG ;

Eisenhauer, E ;

Mirimanoff, RO ;

Van Den Weyngaert, D ;

Kaendler, S ;

Krauseneck, P ;

Vinolas, N ;

Villa, S ;

Wurm, RE ;

Maillot, MHB ;

Spagnolli, F ;

Kantor, G ;

Malhaire, JP ;

Renard, L ;

De Witte, O ;

Scandolaro, L ;

Vecht, CJ ;

Maingon, P ;

Lutterbach, J ;

Kobierska, A ;

Bolla, M ;

Souchon, R ;

Mitine, C ;

Tzuk-Shina, T ;

Kuten, A ;

Haferkamp, G ;

de Greve, J ;

Priou, F ;

Menten, J ;

Rutten, I ;

Clavere, P ;

Malmstrom, A ;

Jancar, B ;

Newlands, E .

NEW ENGLAND JOURNAL OF MEDICINE, 2005, 352 (10) :987-996

[30] Neuronal and glioma-derived stem cell factor induces angiogenesis within the brain [J].

Sun, LX ;

Hui, AM ;

Su, Q ;

Vortmeyer, A ;

Kotliarov, Y ;

Pastorino, S ;

Passaniti, A ;

Menon, J ;

Walling, J ;

Bailey, R ;

Rosenblum, M ;

Mikkelsen, T ;

Fine, HA .

CANCER CELL, 2006, 9 (04) :287-300

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