Overexpression of lipase A and B of Geotrichum candidum in Pichia pastoris: High-level production and some properties of functional expressed lipase B

被引：31

作者：

Catoni, E ^{[1
]}

SchmidtDannert, C ^{[1
]}

Brocca, S ^{[1
]}

Schmid, RD ^{[1
]}

机构：

[1] UNIV STUTTGART, INST TECH BIOCHEM, D-70569 STUTTGART, GERMANY

来源：

BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 09期

关键词：

D O I：

10.1023/A:1018419729009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipase A and B from Geotrichum candidum CMICC 335426 were functionally expressed and secreted in Pichia pastoris. Lipase B exhibits a unique substrate specificity for long-chain cis-Delta 9 unsaturated triacylglycerols. Thus, using different cultivation conditions, the production of recombinant lipase B was optimised, yielding 200 000 Units per liter culture. After overexpression lipase B was purified by diafiltration (372 U/mg). The investigated enzyme properties were comparable to those reported for the native enzyme.

引用

页码：689 / 695

页数：7

共 15 条

[1] POLYMORPHISM IN THE LIPASE GENES OF GEOTRICHUM-CANDIDUM STRAINS [J].

BERTOLINI, MC ;

LARAMEE, L ;

THOMAS, DY ;

CYGLER, M ;

SCHRAG, JD ;

VERNET, T .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 219 (1-2) :119-125

[2]

BERTOLINI MC, 1995, EUR J BIOCHEM, V228, P863, DOI 10.1111/j.1432-1033.1995.0863m.x

[3] LIPASE-CATALYZED SYNTHESES OF MONOACYLGLYCEROLS [J].

BORNSCHEUER, UT .

ENZYME AND MICROBIAL TECHNOLOGY, 1995, 17 (07) :578-586

[4] A COMPARISON OF SILVER STAINING METHODS FOR DETECTING PROTEINS IN ULTRATHIN POLYACRYLAMIDE GELS ON SUPPORT FILM AFTER ISOELECTRIC-FOCUSING [J].

BUTCHER, LA ;

TOMKINS, JK .

ANALYTICAL BIOCHEMISTRY, 1985, 148 (02) :384-388

[5] SUBSTRATE SPECIFICITIES OF LIPASE-A AND LIPASE-B FROM GEOTRICHUM-CANDIDUM CMICC-335426 [J].

CHARTON, E ;

MACRAE, AR .

BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1123 (01) :59-64

[6] SPECIFICITIES OF IMMOBILIZED GEOTRICHUM-CANDIDUM CMICC-335426 LIPASE-A AND LIPASE-B IN HYDROLYSIS AND ESTER SYNTHESIS REACTIONS IN ORGANIC-SOLVENTS [J].

CHARTON, E ;

MACRAE, AR .

ENZYME AND MICROBIAL TECHNOLOGY, 1993, 15 (06) :489-493

[7] LARGE-SCALE PURIFICATION, ENZYMATIC CHARACTERIZATION, AND CRYSTALLIZATION OF THE LIPASE FROM GEOTRICHUM-CANDIDUM [J].

HEDRICH, HC ;

SPENER, F ;

MENGE, U ;

HECHT, HJ ;

SCHMID, RD .

ENZYME AND MICROBIAL TECHNOLOGY, 1991, 13 (10) :840-847

[8] SEPARATION AND CHARACTERIZATION OF 61-KDA AND 57-KDA LIPASES FROM GEOTRICHUM-CANDIDUM ATCC 66592 [J].

JACOBSEN, T ;

POULSEN, OM .

CANADIAN JOURNAL OF MICROBIOLOGY, 1992, 38 (01) :75-80

[9]

Sambrock J., 1989, MOL CLONING LAB MANU

[10] LIPASE-CATALYZED TRANSESTERIFICATION IN ORGANIC-SOLVENTS - APPLICATIONS TO THE PREPARATION OF ENANTIOMERICALLY PURE COMPOUNDS [J].

SANTANIELLO, E ;

FERRABOSCHI, P ;

GRISENTI, P .

ENZYME AND MICROBIAL TECHNOLOGY, 1993, 15 (05) :367-382

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