Pseudomonas coleopterorum sp nov., a cellulase-producing bacterium isolated from the bark beetle Hylesinus fraxini

We isolated a strain coded Esc2Am(T) during a study focused on the microbial diversity of adult specimens of the bark beetle Hylesinus fraxini. Its 16S rRNA gene sequence had 99.4 % similarity with respect to its closest relative, Pseudomonas rhizosphaerae IH5(T). The analysis of partial sequences of the housekeeping genes rpoB, rpoD and gyrB confirmed that strain Esc2Am(T) formed a cluster with P. rhizosphaerae IH5(T) clearly separated from the remaining species of the genus Pseudomonas. Strain Esc2Am(T) had polar flagella and could grow at temperatures from 4 degrees C to 30 degrees C. The respiratory quinone was 09 and the main fatty acids were C-16 : 0, C-18 (:) (1)omega 7c and/or C-18 (:) (1)omega 6c in summed feature 8 and C-16 (:) (1)omega 7c and/or C-18 : 1 omega 6c in summed feature 3. DNA DNA hybridization results showed 51 % relatedness with respect to P. rhizosphaerae IH5(T). Oxidase, catalase and urease-positive, the arginine dihydrolase system was present but nitrate reduction and beta-galactosidase production were negative. Aesculin hydrolysis was positive. Based on the results from the genotypic, phenotypic and chemotaxonomic analyses, we propose the classification of strain Esc2Am(T) as representing a novel species of the genus Pseudomonas, for which we propose the name Pseudomonas coleopterorum sp. nov. The type strain is Esc2Am(T) (=LMG 28558(T)=CECT 8695(T)).