Capsid protein and helper component proteinase function as potyvirus cell-to-cell movement proteins

被引:201
作者
Rojas, MR
Zerbini, FM
Allison, RF
Gilbertson, RL
Lucas, WJ
机构
[1] UNIV CALIF DAVIS, DIV BIOL SCI, PLANT BIOL SECT, DAVIS, CA 95616 USA
[2] MICHIGAN STATE UNIV, DEPT BOT & PLANT PATHOL, E LANSING, MI 48824 USA
[3] UNIV CALIF DAVIS, DEPT PLANT PATHOL, DAVIS, CA 95616 USA
[4] UNIV CALIF DAVIS, DEPT AGRON & RANGE SCI, DAVIS, CA 95616 USA
基金
美国国家科学基金会;
关键词
D O I
10.1006/viro.1997.8777
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The role of bean common mosaic necrosis potyvirus (BCMNV) and lettuce mosaic potyvirus (LMV) proteins was investigated in terms of their capacity to function as viral movement proteins (MPs). Using Escherichia coli-expressed proteins and microinjection techniques, direct evidence was obtained that both the potyviral capsid protein (CP) and helper component-proteinase (HC-Pro) function in this capacity, in that both proteins (a) trafficked from cell to cell, (b) induced an increase in plasmodesmal size exclusion limit, and (c) facilitated cell-to-cell movement of viral RNA. CP and HC-Pro mutants were also produced and used in microinjection experiments. Mutations in the core region of the CP either impaired (single and double amino acid substitution mutants) or abolished (triple amino acid substitution mutant) cell-to-cell movement, as did C-terminal deletion mutants in HC-Pro. The BCMNV P1, Cl, Nla, and Nlb proteins did not exhibit viral MP properties, but Nla and Nlb proteins were found to accumulate within the nuclei of injected cells. These results further establish the multifunctional nature of the potyvirus CP and HC-Pro. (C) 1997 Academic Press.
引用
收藏
页码:283 / 295
页数:13
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