Highly efficient inhibition of human immunodeficiency virus type 1 reverse transcriptase by aptamers functionalized gold nanoparticles

被引：42

作者：

Shiang, Yen-Chun ^{[1
]}

Ou, Chung-Mao ^{[1
]}

Chen, Shih-Ju ^{[1
]}

Ou, Ting-Yu ^{[2
,3
]}

Lin, Han-Jia ^{[2
,3
]}

Huang, Chih-Ching ^{[2
,3
]}

Chang, Huan-Tsung ^{[1
]}

机构：

[1] Natl Taiwan Univ, Dept Chem, Taipei 10617, Taiwan

[2] Natl Taiwan Ocean Univ, Inst Biosci & Biotechnol, Keelung 20224, Taiwan

[3] Natl Taiwan Ocean Univ, Ctr Excellence Marine Bioenvironm & Biotechnol CM, Keelung 20224, Taiwan

来源：

NANOSCALE | 2013年 / 5卷 / 07期

关键词：

TARGETED CANCER-THERAPY; DRUG-RESISTANCE; HIV-1; PROTEASE; NUCLEIC-ACIDS; DNA APTAMERS; C VIRUS; CELLS; ENTRY; ASSAY; NANOTECHNOLOGY;

D O I：

10.1039/c3nr33403a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have developed aptamer (Apt)-conjugated gold nanoparticles (Apt-Au NPs, 13 nm in diameter) as highly effective inhibitors for human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT). Two Apts, RT1t49 (Apt(pol)) and ODN 93 (Apt(RH)), which recognize the polymerase and RNase H regions of HIV-1 RT, are used to conjugate Au NPs to prepare Apt(pol)-Au NPs and Apt(RH)-Au NPs, respectively. In addition to DNA sequence, the surface density of the aptamers on Au NPs (nApt-Au NPs; n is the number of aptamer molecules on each Au NP) and the linker length number (T-m; m is the base number of the deoxythymidine linker) between the aptamer and Au NPs play important roles in determining their inhibition activity. A HIV-lentiviral vector-based antiviral assay has been applied to determine the inhibitory effect of aptamers or Apt-Au NPs on the early stages of their replication cycle. The nucleasestable G-quadruplex structure of 40Apt(RH)-T-45-Au NPs shows inhibitory efficiency in the retroviral replication cycle with a decreasing infectivity (40.2%).

引用

页码：2756 / 2764

页数：9

共 90 条

[1] DNA aptamers selected against the HIV-1 RNase H display in vitro antiviral activity [J].