Fabrication of Supported Lipid Bilayer on Graphene Oxide

被引：26
|
作者：
Okamoto, Y. [1 ]
Tsuzuki, K. [1 ]
Iwasa, S. [1 ]
Ishikawa, R. [2 ]
Sandhu, A. [2 ,3 ]
Tero, R. [3 ]
机构：
[1] Toyohashi Univ Technol, Dept Environm & Life Sci, 1-1 Hibarigaoka,Tempaku Cho, Toyohashi, Aichi 4418580, Japan
[2] Tokyo Inst Technol, Dept Elect & Elect Engn, Tokyo 1528552, Japan
[3] Toyohashi Univ Technol, Elect Inspired Interdisciplinary Res Inst, Toyohashi, Aichi 4418520, Japan
来源：
ASIA-PACIFIC INTERDISCIPLINARY RESEARCH CONFERENCE 2011 (AP-IRC 2011) | 2012年 / 352卷
关键词：
SILICON DIOXIDE SURFACES; PHOSPHOLIPID-BILAYERS; CELL BIOLOGY; MEMBRANE; RAFTS; PROTEINS; AFM; PRINCIPLE; PLATFORM; DENSITY;
D O I：
10.1088/1742-6596/352/1/012017
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
Planar lipid bilayers of dioleoylphosphatidylcholine was prepared on graphene oxide (GO), as a fundamental platform for biosensing in plasma membrane model using graphene. The GO flakes were prepared according to modified Hummer's method, and deposited on thermally oxidized SiO2/Si surfaces. We found that planar lipid bilayers were reproducibility formed on the GO/SiO2/Si surface in the presence of Ca2+ ions, while unruptured vesicles remained on the GO surface without Ca2+ ion. The results of atomic force microscope observation and fluorescence recovery after photobleaching experiment revealed that double lipid bilayers were spontaneously formed on the GO surfaces.
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页数：6
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