MOLECULAR CHARACTERISATION OF EPIZOOTIC HAEMORRHAGIC DISEASE VIRUS ASSOCIATED WITH A TUNISIAN OUTBREAK AMONG CATTLE IN 2006

被引：18

作者：

Ben Dhaou, Sameh ^{[1
,2
,4
]}

Sailleau, Corinne ^{[1
]}

Babay, Besma ^{[2
]}

Viarouge, Cyril ^{[1
]}

Sghaier, Soufien ^{[2
]}

Zientara, Stephan ^{[1
]}

Hammami, Salah ^{[2
,3
]}

Breard, Emmanuel ^{[1
]}

机构：

[1] Anses, ENVA, INRA, UPEC,UMR Virol 1161, 23 Ave Gen Gaulle, F-94706 Maisons Alfort, France

[2] IRVT, Tunis, Tunisia

[3] ENMV, Tunis, Tunisia

[4] Fac Sci Bizerte, Jarzouna Bizerte, Tunisia

来源：

ACTA VETERINARIA HUNGARICA | 2016年 / 64卷 / 02期

关键词：

Epizootic haemorrhagic disease virus; serotype; 6; RT-PCR; sequencing; phylogenetic tree; BLUETONGUE VIRUS; CAPSID PROTEIN; DEER VIRUS; SEROTYPES; ANTIBODY; GENOME;

D O I：

10.1556/004.2016.025

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

In 2006, epizootic haemorrhagic disease (EHD) outbreaks were recorded in the Maghreb (Tunisia, Morocco and Algeria) among cattle, resulting in severe repercussions on herds (oedema of the head, necrotic lesions of the oral mucosa, hyperthermia of the teats, accompanied by anorexia and respiratory distress) and economic losses. The present study gives new information on the molecular characterisation of the EHD virus (EHDV) that had circulated in Tunisia. Genome segments 2, 3, 6, 7 and 10 of EHDV, corresponding to the VP2, VP3, VP5, VP7 and NS3/NS3A proteins, respectively, were amplified from the blood of one animal by RT-PCR and sequenced. Nucleotide sequence comparisons of these five segments with sequences available in the GenBank demonstrated that an EHDV serotype 6 (EHDV-6) had been present in Tunisia in 2006. The possible origin of this strain is discussed.

引用

页码：250 / 262

页数：13

共 34 条

[1] Genetic and phylogenetic analysis of the core proteins VP1, VP3, VP4, VP6 and VP7 of epizootic haemorrhagic disease virus (EHDV) [J].

Anthony, S. J. ;

Maan, N. ;

Maan, S. ;

Sutton, G. ;

Attoui, H. ;

Mertens, P. P. C. .

VIRUS RESEARCH, 2009, 145 (02) :187-199

[2] Genetic and phylogenetic analysis of the outer-coat proteins VP2 and VP5 of epizootic haemorrhagic disease virus (EHDV): Comparison of genetic and serological data to characterise the EHDV serogroup [J].

Anthony, S. J. ;

Maan, S. ;

Maan, N. ;

Kgosana, L. ;

Bachanek-Bankowska, K. ;

Batten, C. ;

Darpel, K. E. ;

Sutton, G. ;

Attoui, H. ;

Mertens, P. P. C. .

VIRUS RESEARCH, 2009, 145 (02) :200-210

[3] Genetic and phylogenetic analysis of the non-structural proteins NS1, NS2 and NS3 of epizootic haemorrhagic disease virus (EHDV) [J].

Anthony, S. J. ;

Maan, N. ;

Maan, S. ;

Sutton, G. ;

Attoui, H. ;

Mertens, P. P. C. .

VIRUS RESEARCH, 2009, 145 (02) :211-219

[4] Outbreak of epizootic haemorrhagic disease on the island of Reunion [J].

Bréard, E ;

Sailleau, C ;

Hamblin, C ;

Graham, SD ;

Gourreau, JM ;

Zientara, S .

VETERINARY RECORD, 2004, 155 (14) :422-+

[5] Epizootic hemorrhagic disease virus serotype 6 experimentation on adult cattle [J].

Breard, Emmanuel ;

Belbis, Guillaume ;

Viarouge, Cyril ;

Riou, Mickael ;

Desprat, Alexandra ;

Moreau, Joel ;

Laloy, Eve ;

Martin, Guillaume ;

Sarradin, Pierre ;

Vitour, Damien ;

Batten, Carrie ;

Doceul, Virginie ;

Sailleau, Corinne ;

Zientara, Stephan .

RESEARCH IN VETERINARY SCIENCE, 2013, 95 (02) :794-798

[6] GEOGRAPHICAL GENETIC-VARIATION IN THE GENE ENCODING VP3 FROM THE ALBERTA ISOLATE OF EPIZOOTIC HEMORRHAGIC-DISEASE VIRUS [J].

CHENEY, IW ;

LARSON, MD ;

MECHAM, JO ;

WILSON, WC .

VIRUS RESEARCH, 1995, 36 (2-3) :279-286

[7]

European Food Safety Authority, 2007, SCI OP EP HEM DIS

[8]

Hammami S., 2007, B VET I RECHERCHE VE, V25, P6

[9] CLUSTAL - A PACKAGE FOR PERFORMING MULTIPLE SEQUENCE ALIGNMENT ON A MICROCOMPUTER [J].

HIGGINS, DG ;

SHARP, PM .

GENE, 1988, 73 (01) :237-244

[10]

Huismans H, 2004, Vet Ital, V40, P417

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