Chemical initiation for butyl and lauryl acrylate monolithic columns for CEC

被引：16

作者：

Canto-Mirapeix, Amparo ^{[1
]}

Herrero-Martinez, Jose M. ^{[1
]}

Mongay-Fernandez, Carlos ^{[1
]}

Simo-Alfonso, Ernesto F. ^{[1
]}

机构：

[1] Univ Valencia, Fac Chem, Dept Analyt Chem, E-46100 Valencia, Spain

来源：

ELECTROPHORESIS | 2009年 / 30卷 / 04期

关键词：

Acrylate ester-based monolithic columns; Ammonium peroxodisulfate; Butyl acrylate; Lauroyl peroxide; Lauryl acrylate; PHASE LIQUID-CHROMATOGRAPHY; HIGH-FLOW CHARACTERISTICS; POROUS POLYMER MONOLITHS; IN-SITU SYNTHESIS; STATIONARY PHASES; CAPILLARY ELECTROCHROMATOGRAPHY; SEPARATION MEDIA; MACROPOROUS POLYMER; AMINO-ACIDS; METHACRYLATE;

D O I：

10.1002/elps.200800678

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Butyl acrylate (BA)- and lauryl acrylate (LA)-based monolithic stationary phases for CEC were synthesized, using a redox system as initiator of polymerization. BA monoliths were initiated with ammonium peroxodisulfate, whereas LA columns were obtained with lauroyl peroxide as initiator. In both cases, TEMED was used to activate the process. The influence of porogenic solvent composition on both morphological and electrochromatographic properties of the resulting monoliths was investigated. Excellent efficiencies (minimum plate heights of 4.2-6.3 mu m for BA columns and 2.6-5.3 mu m for LA stationary phases, for a PAHs mixture) were achieved. The capability of separation of both types of monolithic beds was compared by the analysis of complex mixtures of polycyclic aromatic hydrocarbons and anabolic steroids.

引用

页码：599 / 606

页数：8

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