Monitoring angiogenesis noninvasively with near-infrared spectroscopy

被引：10

作者：

Dunn, Jeffrey F. ^{[1
,2
,3
]}

Zhang, Qiong ^{[1
,2
]}

Wu, Ying ^{[1
,2
,3
]}

Srinivasan, Sathyanarayanan ^{[1
,2
,3
]}

Smith, Michael R. ^{[4
]}

Shaw, R. Anthony ^{[5
]}

机构：

[1] Univ Calgary, Fac Med, Dept Radiol, Calgary, AB T2N 4N1, Canada

[2] Univ Calgary, Expt Imaging Ctr, Calgary, AB T2N 4N1, Canada

[3] Univ Calgary, Hotchkiss Brain Inst, Calgary, AB T2N 4N1, Canada

[4] Univ Calgary, Dept Elect & Comp Engn, Calgary, AB T2N 4N1, Canada

[5] NRC Inst Biodiagnost, Winnipeg, MB R3B 1Y6, Canada

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2008年 / 13卷 / 06期

基金：

加拿大健康研究院;

关键词：

near-infrared spectroscopy; cerebral blood volume; angiogenesis; hypoxia; anoxia; brain;

D O I：

10.1117/1.3000431

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Near-infrared (NIR) spectroscopy is used to quantify cerebral blood volume (CBV) as a marker of angiogenesis (formation of new blood vessels). Rats are exposed to chronic hypoxia for 3 weeks at half atmospheric pressure to stimulate angiogenesis, and second-differential NIR spectroscopy is used to quantify total cerebral hemoglobin before and after angiogenesis. The cerebral hemoglobin (from broadband NIR spectroscopy), and the large vessel hemoglobin and hematocrit (from blood samples), are used to derive values for the calculation of CBV. The total hemoglobin in brain is 46.6 +/- 1.9 mu mol/l (mean +/- SD, n = 5) preacclimation and increases by 72% postacclimation. CBV is initially 3.26 +/- 0.41% v/v and increases by 31% with acclimation. Each individual animal shows a measureable increase in CBV. This study indicates that NIR broadband spectroscopy can be used for repeated measurements of CBV and can be applied as a noninvasive method to study angiogenesis. (c) 2008 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3000431]

引用

页数：4

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