Crosstalk between endoplasmic reticulum stress and oxidative stress in apoptosis induced by α-tocopheryl succinate in human gastric carcinoma cells

被引：18

作者：

Huang, Xiaoli ^{[1
,2
]}

Li, Li ^{[3
]}

Zhang, Linyou ^{[3
]}

Zhang, Zhihong ^{[1
]}

Wang, Xiaolin ^{[1
]}

Zhang, Xuguang ^{[1
]}

Hou, Liying ^{[1
]}

Wu, Kun ^{[1
]}

机构：

[1] Harbin Med Univ, Dept Nutr & Food Hyg, Harbin 150081, Peoples R China

[2] Shandong Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Jinan 250100, Peoples R China

[3] Harbin Med Univ, Affiliated Hosp 2, Harbin, Peoples R China

来源：

BRITISH JOURNAL OF NUTRITION | 2013年 / 109卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Apoptosis; Endoplasmic reticulum stress; Oxidative stress; alpha-Tocopheryl succinate; C/EBP homologous protein transcription factor; UNFOLDED PROTEIN RESPONSE; SPECIES-DEPENDENT MECHANISM; RESPIRATORY COMPLEX-II; CELLULAR REDOX STATE; CANCER CELLS; ER STRESS; DEATH; INDUCTION; ANTIOXIDANTS; PATHWAYS;

D O I：

10.1017/S0007114512001882

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

alpha-Tocopheryl succinate (alpha-TOS) has been shown to be a potent apoptosis inducer and growth inhibitor in a variety of cancer cells. Our previous studies showed the important role of endoplasmic reticulum (ER) stress and reactive oxygen species (ROS) generation in the apoptosis induced by alpha-TOS. However, the relationship of oxidative stress with ER stress is still controversial. The objective of the present study was to investigate the interplay between the two stress responses induced by alpha-TOS in SGC-7901 human gastric cancer cells. In response to alpha-TOS, cytological changes typical of apoptosis, induction of glucose-regulated protein 78 (GRP78) and CCAAT/enhancer-binding protein (C/EBP) homologous protein transcription factor (CHOP), and activation of caspase-4 were observed. And the antioxidant N-acetyl-L-cysteine inhibited induction of both GRP78 and CHOP by alpha-TOS transcriptionally and translationally. Furthermore, knocking down CHOP by RNA interference decreased ROS generation, increased glutathione level and induced glutathione peroxidase mRNA expression in alpha-TOS-treated cells, whereas catalase and superoxide dismutases mRNA expression were not altered. The results imply that alpha-TOS induces ER stress response through ROS production, while CHOP perturbs the redox state of SGC-7901 cells treated with alpha-TOS.

引用

页码：727 / 735

页数：9

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