Chip-based genotyping by mass spectrometry

被引：214

作者：

Tang, K ^{[1
]}

Fu, DJ ^{[1
]}

Julien, D ^{[1
]}

Braun, A ^{[1
]}

Cantor, CR ^{[1
]}

Köster, H ^{[1
]}

机构：

[1] Sequenom Inc, San Diego, CA 92121 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1999年 / 96卷 / 18期

关键词：

DNA chip; single nucleotide polymorphism;

D O I：

10.1073/pnas.96.18.10016

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Silicon chips with immobilized target DNAs were used for accurate genotyping by mass spectrometry. Genomic DNAs were amplified with PCR, and the amplified products were covalently attached to chip wells via N-succinimidyl (4-iodoacetyl)aminobenzoate (SLAB) chemistry. Primer annealing, extension, and termination were performed on a 1-mu l scale directly in the chip wells in parallel. Diagnostic products thus generated were detected in situ by using matrix-assisted laser desorption ionization mass spectrometry, This miniaturized method has the potential for accurate, high-throughput, low-cost identification of genetic variations.

引用

收藏

页码：10016 / 10020

页数：5

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