Co-upregulation of Toll-like receptors 2 and 6 on peripheral blood cells in patients with obstructive sleep apnea

被引：21

作者：

Chen, Yung-Che ^{[1
,2
,3
,8
]}

Su, Mao-Chang ^{[1
,2
,3
,9
]}

Liou, Chia-Wei ^{[2
,4
]}

Liu, Shih-Feng ^{[1
,2
,5
]}

Chen, Chung-Jen ^{[2
,6
]}

Lin, Hsin-Ching ^{[2
,3
,7
]}

Hsiao, Chang-Chun ^{[8
]}

Wang, Ting-Ya ^{[1
,2
]}

Wang, Chin-Chou ^{[1
,2
,3
,9
]}

Chin, Chien-Hung ^{[1
,2
,3
]}

Huang, Kuo-Tung ^{[1
,2
]}

Lin, An-Shen ^{[1
,2
]}

Lin, Meng-Chih ^{[1
,2
,3
,8
,10
]}

机构：

[1] Kaohsiung Chang Gung Mem Hosp, Div Pulm & Crit Care Med, Kaohsiung, Taiwan

[2] Chang Gung Univ, Coll Med, Kaohsiung, Taiwan

[3] Kaohsiung Chang Gung Mem Hosp, Sleep Ctr, Kaohsiung, Taiwan

[4] Kaohsiung Chang Gung Mem Hosp, Dept Neurol, Kaohsiung, Taiwan

[5] Kaohsiung Chang Gung Mem Hosp, Dept Resp Therapy, Kaohsiung, Taiwan

[6] Kaohsiung Chang Gung Mem Hosp, Div Rheumatol, Kaohsiung, Taiwan

[7] Kaohsiung Chang Gung Mem Hosp, Dept Otolaryngol, Kaohsiung, Taiwan

[8] Chang Gung Univ, Coll Med, Grad Inst Clin Med Sci, Taoyuan, Taiwan

[9] Chang Gung Univ Sci & Technol, Dept Resp Care, Chiayi, Taiwan

[10] Kaohsiung Chang Gung Mem Hosp, Dept Med, Div Pulm & Crit Care Med, Kaohsiung, Taiwan

来源：

SLEEP AND BREATHING | 2015年 / 19卷 / 03期

关键词：

Toll-like receptor 2; Toll-like receptor 6; Obstructive sleep apnea; Intermittent hypoxia with re-oxygenation; NF-KAPPA-B; NEUTROPHIL APOPTOSIS; INTERMITTENT HYPOXIA; MOLECULAR PATHWAYS; EPITHELIAL-CELLS; ACTIVATION; TLR2; MACROPHAGES; LIPOPROTEIN; EXPRESSION;

D O I：

10.1007/s11325-014-1116-4

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Toll-like receptor (TLR) 2 can heterodimerise with TLR6 to detect diacylated lipoproteins. Hypoxia inducible factor-1 alpha co-ordinates selective induction of TLR2 and TLR6 during persistent hypoxia. We hypothesized that TLR 2/6 co-expression may be upregulated by chronic intermittent hypoxia with re-oxygenation (IHR) in obstructive sleep apnea (OSA). TLR2/6 expressions on blood immune cells were measured in 144 patients with sleep-disordered breathing (SDB), including primary snoring (PS, n = 24), moderate to severe OSA (MSO, n = 60), very severe OSA (VSO, n = 36), and very severe OSA on continuous positive airway pressure (CPAP) treatment (VSOC, n = 24). An in vitro IHR experiment was also undertaken. Patients in both the MSO and VSO groups had increased TLR2/6 co-expression on CD16(+) neutrophil than those in the PS group. Patients in the VSOC group had reduced TLR2/6 co-expression on neutrophil than those in either the MSO or VSO group. Blood absolute neutrophil count was positively but weakly correlated with TLR2/6 co-expression on neutrophil. TLR2/6 co-expressions on both CD14(+) monocyte and CD3(+)CD4(+)T helper cell, and TLR2 expressions on both monocyte and T helper cell in SDB patients with low Minimum SaO(2) (a parts per thousand broken vertical bar 70 %) were all higher than those with high Minimum SaO(2). In vitro IHR for 1-4 days resulted in TLR2/6 co-upregulation on both neutrophil and monocyte. OSA patients had increased TLR2/6 co-expressions on blood immune cells, which were related to their immune cell counts and could be reversed with CPAP treatment. In vitro IHR could induce TLR2/6 co-upregulation.

引用

页码：873 / 882

页数：10

共 37 条

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