Development of a transposon mutagenesis system in the oral spirochete Treponema denticola

被引：18
|
作者：
Yang, Yu [1 ]
Stewart, Philip E. [2 ]
Shi, Xiaoguang [1 ]
Li, Chunhao [1 ]
机构：
[1] SUNY Buffalo, Dept Oral Biol, Buffalo, NY 14214 USA
[2] NIAID, Lab Zoonot Pathogens, Rocky Mt Labs, NIH, Hamilton, MT 59840 USA
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 20期
关键词：
D O I：
10.1128/AEM.01424-08
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Here, we report successful transposon mutagenesis in the oral spirochete Treponema denticola. A modified Himar1 transposon, including a new antibiotic selection cassette for T. denticola, generated mutations affecting cell division, transport, and chemotaxis, among other processes. This random mutagenesis system should facilitate research on the biology and pathogenesis of this spirochete, which is associated with human periodontal diseases.
引用
收藏
页码：6461 / 6464
页数：4
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