Expression profiles of miRNAs in polarized macrophages

被引：185

作者：

Zhang, Yingying ^{[1
]}

Zhang, Mengying ^{[2
]}

Zhong, Min ^{[2
]}

Suo, Qifeng ^{[2
]}

Lv, Kun ^{[2
]}

机构：

[1] Yijishan Hosp, Wannan Med Coll, Lab Med, Wuhu 241001, Anhui, Peoples R China

[2] Yijishan Hosp, Wannan Med Coll, Cent Lab, Wuhu 241001, Anhui, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2013年 / 31卷 / 04期

关键词：

polarization; bone marrow-derived macrophages; microRNA; microarray; ALTERNATIVE ACTIVATION; INFLAMMATORY RESPONSE; INTERFERON-GAMMA; MICRORNA; GENES; CELLS; DIFFERENTIATION; STIMULATION; INDUCTION; MONOCYTES;

D O I：

10.3892/ijmm.2013.1260

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

microRNAs (miRNAs) play a crucial role in tissue development and the pathology of various diseases. However, the effects and roles of miRNAs in macrophage polarization have yet to be investigated. In this study, we analyzed and compared the miRNA expression profiles of bone marrow-derived macrophages (BMDMs) with two distinct polarizing conditions (classical macrophage activation 'MI' and alternative activation 'M2') using miRNA microarray. In total, 109 miRNAs were differentially expressed between M1 and M2. The differential expression of selected miRNAs was validated by real-time qRT-PCR: miR-181a, miR-155-5p, miR-204-5p and miR-451 were upregulated (fold change >2, P<0.05) and miR-125-5p, miR-146a-3p, miR-143-3p and miR-145-5p were downregulated (fold change <-2, P<0.05) in M1 compared with M2. In conclusion, our study may be useful for exploring the precise roles of miRNAs in macrophage differentiation and polarized activation processes in the future.

引用

页码：797 / 802

页数：6

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