Solid Plate-based Dietary Restriction in Caenorhabditis elegans

被引:14
作者
Ching, Tsui-Ting [1 ]
Hsu, Ao-Lin [1 ,2 ]
机构
[1] Univ Michigan, Dept Internal Med, Div Geriatr Med, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Mol & Integrat Physiol, Ann Arbor, MI 48109 USA
来源
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2011年 / 51期
关键词
Developmental Biology; Issue; 51; Dietary restriction; caloric restriction; C; elegans; longevity;
D O I
10.3791/2701
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Reduction of food intake without malnutrition or starvation is known to increase lifespan and delay the onset of various age-related diseases in a wide range of species, including mammals. It also causes a decrease in body weight and fertility, as well as lower levels of plasma glucose, insulin, and IGF-1 in these animals. This treatment is often referred to as dietary restriction (DR) or caloric restriction (CR). The nematode Caenorhabditis elegans has emerged as an important model organism for studying the biology of aging. Both environmental and genetic manipulations have been used to model DR and have shown to extend lifespan in C. elegans. However, many of the reported DR studies in C. elegans were done by propagating animals in liquid media, while most of the genetic studies in the aging field were done on the standard solid agar in petri plates. Here we present a DR protocol using standard solid NGM agar-based plate with killed bacteria.
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页数:4
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共 15 条
  • [1] Regulation of lifespan by sensory perception in Caenorhabditis elegans
    Apfeld, J
    Kenyon, C
    [J]. NATURE, 1999, 402 (6763) : 804 - 809
  • [2] Two neurons mediate diet-restriction-induced longevity in C-elegans
    Bishop, Nicholas A.
    Guarente, Leonard
    [J]. NATURE, 2007, 447 (7144) : 545 - +
  • [3] drr-2 encodes an eIF4H that acts downstream of TOR in diet-restriction-induced longevity of C-elegans
    Ching, Tsui-Ting
    Paal, Alisha B.
    Mehta, Avni
    Zhong, Linda
    Hsu, Ao-Lin
    [J]. AGING CELL, 2010, 9 (04) : 545 - 557
  • [4] An AMPK-FOXO pathway mediates longevity induced by a novel method of dietary restriction in C-elegans
    Greer, Eric L.
    Dowlatshahi, Dara
    Banko, Max R.
    Villen, Judit
    Hoang, Kimmi
    Blanchard, Daniel
    Gygi, Steven P.
    Brunet, Anne
    [J]. CURRENT BIOLOGY, 2007, 17 (19) : 1646 - 1656
  • [5] No reduction of metabolic rate in food restricted Caenorhabditis elegans
    Houthoofd, K
    Braeckman, BP
    Lenaerts, I
    Brys, K
    De Vreese, A
    Van Eygn, S
    Vanfleteren, JR
    [J]. EXPERIMENTAL GERONTOLOGY, 2002, 37 (12) : 1359 - 1369
  • [6] Axenic growth up-regulates mass-specific metabolic rate, stress resistance, and extends life span in Caenorhabditis elegans
    Houthoofd, K
    Braeckman, BP
    Lenaerts, I
    Brys, K
    De Vreese, A
    Van Eygen, S
    Vanfleteren, JR
    [J]. EXPERIMENTAL GERONTOLOGY, 2002, 37 (12) : 1371 - 1378
  • [7] Regulation of aging and age-related disease by DAF-16 and heat-shock factor
    Hsu, AL
    Murphy, CT
    Kenyon, C
    [J]. SCIENCE, 2003, 300 (5622) : 1142 - 1145
  • [8] Lifespan extension in Caenorhabditis elegans by complete removal of food
    Kaeberlein, Tammi L.
    Smith, Erica D.
    Tsuchiya, Mitsuhiro
    Welton, K. Linnea
    Thomas, James H.
    Fields, Stanley
    Kennedy, Brian K.
    Kaeberlein, Matt
    [J]. AGING CELL, 2006, 5 (06) : 487 - 494
  • [9] A C-ELEGANS MUTANT THAT LIVES TWICE AS LONG AS WILD-TYPE
    KENYON, C
    CHANG, J
    GENSCH, E
    RUDNER, A
    TABTIANG, R
    [J]. NATURE, 1993, 366 (6454) : 461 - 464
  • [10] AGING IN NEMATODE CAENORHABDITIS-ELEGANS - MAJOR BIOLOGICAL AND ENVIRONMENTAL-FACTORS INFLUENCING LIFE-SPAN
    KLASS, MR
    [J]. MECHANISMS OF AGEING AND DEVELOPMENT, 1977, 6 (06) : 413 - 429