The relevance of a digestibility evaluation in the allergenicity risk assessment of novel proteins. Opinion of a joint initiative of COST action ImpARAS and COST action INFOGEST

被引：59

作者：

Verhoeckx, Kitty ^{[1
]}

Bogh, Katrine Lindholm ^{[2
]}

Dupont, Didier ^{[3
]}

Egger, Lotti ^{[4
]}

Gadermaier, Gabriele ^{[5
]}

Larre, Colette ^{[6
]}

Mackie, Alan ^{[7
]}

Menard, Olivia ^{[1
]}

Adel-Patient, Karine ^{[8
]}

Picariello, Gianluca ^{[9
]}

Portmann, Reto ^{[10
]}

Smit, Joost ^{[11
]}

Turner, Paul ^{[12
]}

Untersmayr, Eva ^{[13
]}

Epstein, Michelle M. ^{[14
]}

机构：

[1] TNO, Utrechtseweg 48, Zeist, Netherlands

[2] Tech Univ Denmark, Natl Food Inst, DK-2800 Lyngby, Denmark

[3] INRA Agrocampus Quest, STLO, F-35042 Rennes, France

[4] Agroscope, Schwarzenburgstr 161, CH-3003 Charlotte, Switzerland

[5] Univ Salzburg, Dept Biosci, Hellbrunnerstr 34, A-5020 Salzburg, Austria

[6] INRA UR1268 BIA, Rue Geraudiere,BP 71627, F-44316 Nantes, France

[7] Univ Leeds, Sch Food Sci & Nutr, Leeds LS2 9JT, W Yorkshire, England

[8] Univ Paris Saclay, UMR Serv Pharmacol & Immunoanal, Lab Immunoallergie Alimentaire, CEA,INRA, F-91191 Gif Sur Yvette, France

[9] CNR, Inst Food Sci, Via Roma 64, I-83100 Avellino, Italy

[10] Agroscope, Schwarzenburgstr 161, CH-3003 Bern, Switzerland

[11] Univ Utrecht, Inst Risk Assessment Sci, Yalelaan 104, NL-3584 CM Utrecht, Netherlands

[12] Imperial Coll London, Sect Paediat, London, England

[13] Med Univ Vienna, Ctr Pathophysiol Infectiol & Immunol, Inst Pathophysiol & Allergy Res, Waehringer Guertel 18-20, A-1090 Vienna, Austria

[14] Med Univ Vienna, Dept Dermatol, Expt Allergy Lab, Waehringer Guertel 18-20 Room 4P9-02, A-1090 Vienna, Austria

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2019年 / 129卷

关键词：

Food allergy; Food digestion; Allergenicity risk assessment; Novel proteins; In vitro protein digestibility tests; LIPID TRANSFER PROTEIN; ARA H 1; INTESTINAL EPITHELIAL-CELLS; BOVINE BETA-LACTOGLOBULIN; HYDROLYZED WHEAT-PROTEIN; SIMULATED GASTRIC FLUID; IGE-BINDING ACTIVITY; FREE MOUSE MODEL; IN-VITRO MODEL; PRU P 3;

D O I：

10.1016/j.fct.2019.04.052

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The current allergenicity assessment of novel proteins is based on the EFSA GMO guidance. Recently, EFSA launched a new guidance document on allergenicity assessment of GM plants (2017), This document describes, amongst other topics, the new scientific and regulatory developments on in vitro protein digestibility tests. The EFSA GMO Panel stated that for in vitro protein digestibility tests, additional investigations are needed before any additional recommendation in the form of guidance can be provided. To this end, an interim phase is considered necessary to evaluate the revisions to the in vitro gastrointestinal digestion test, proposed by EFSA. This prompted the establishment of a joint workshop through two COST Action networks: COST Action ImpARAS and COST Acton INFOGEST. In 2017, a workshop was organised to discuss the relevance of digestion in allergenicity risk assessment and how to potentially improve the current methods and readouts. The outcome of the workshop is that there is no rationale for a clear readout that is predictive for allergenicity and we suggest to omit the digestion test from the allergenicity assessment strategy for now, and put an effort into filling the knowledge gaps as summarized in this paper first.

引用

页码：405 / 423

页数：19

共 205 条

[81] Identification of Proteases and Protease Inhibitors in Allergenic and Non-Allergenic Pollen [J].