The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device

被引：226

作者：

Baker, Alexander N. ^{[1
]}

Richards, Sarah-Jane ^{[1
]}

Guy, Collette S. ^{[1
,2
]}

Congdon, Thomas R. ^{[1
]}

Hasan, Muhammad ^{[1
]}

Zwetsloot, Alexander J. ^{[3
]}

Gallo, Angelo ^{[1
]}

Lewandowski, Jozef R. ^{[1
]}

Stansfeld, Phillip J. ^{[1
,2
]}

Straube, Anne ^{[3
]}

Walker, Marc ^{[4
]}

Chessa, Simona ^{[5
]}

Pergolizzi, Giulia ^{[5
]}

Dedola, Simone ^{[5
]}

Field, Robert A. ^{[5
,6
,7
]}

Gibson, Matthew, I ^{[1
,3
]}

机构：

[1] Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England

[2] Univ Warwick, Sch Life Sci, Coventry CV4 7AL, W Midlands, England

[3] Univ Warwick, Warwick Med Sch, Coventry CV4 7AL, W Midlands, England

[4] Univ Warwick, Dept Phys, Coventry CV4 7AL, W Midlands, England

[5] Iceni Diagnost Ltd, Norwich NR4 7GJ, Norfolk, England

[6] Univ Manchester, Dept Chem, Manchester M1 7DN, Lancs, England

[7] Univ Manchester, Manchester Inst Biotechnol, Manchester M1 7DN, Lancs, England

来源：

ACS CENTRAL SCIENCE | 2020年 / 6卷 / 11期

基金：

英国惠康基金; 英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

GOLD NANOPARTICLES; RECEPTOR; CORONAVIRUS; SPECIFICITY; STABILITY; TESTS; ASSAY; COV;

D O I：

10.1021/acscentsci.0c00855

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

There is an urgent need to understand the behavior of of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.

引用

页码：2046 / 2052

页数：7

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