Neural driven angiogenesis by overexpression of nerve growth factor

被引:49
作者
Hansen-Algenstaedt, N.
Algenstaedt, P.
Schaefer, C.
Hamann, A.
Wolfram, L.
Cingoz, G.
Kilic, N.
Schwarzloh, B.
Schroeder, M.
Joscheck, C.
Wiesner, L.
Ruether, W.
Ergun, S.
机构
[1] Univ Hamburg, Hosp Eppendorf, Ctr Med, Dept Orthoped Surg, D-20246 Hamburg, Germany
[2] Univ Hamburg, Hosp Eppendorf, Ctr Med, Dept Internal Med, D-20246 Hamburg, Germany
[3] Univ Hamburg, Hosp Eppendorf, Ctr Med, Dept Anat, D-20246 Hamburg, Germany
[4] Univ Hamburg, Hosp Eppendorf, Ctr Med, Ctr Biomech, D-20246 Hamburg, Germany
[5] Heidelberg Univ, Ctr Med, Dept Internal Med 1, D-69115 Heidelberg, Germany
关键词
angiogenesis; neurogenesis; intravitalmicroscopy; microcirculation;
D O I
10.1007/s00418-005-0111-z
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Mechanisms regulating angiogenesis are crucial in adjusting tissue perfusion on metabolic demands. We demonstrate that overexpression of nerve growth factor (NGF) in brown adipose tissue (BAT) of NGF-transgenic mice elevates both mRNA and protein levels of vascular endothelial growth factor (VEGF) and VEGF-receptors. Increased vascular permeability, leukocyte-endothelial interactions (LEI), and tissue perfusion were measured using intravital microscopy. NGF-stimulation of adipocytes and endothelial cells elevates mRNA expression of VEGF and its receptors, an effect blocked by NGF neutralizing antibodies. These data suggest an activation of angiogenesis as a result of both: stimulation of adipozytes and direct mitogenic effects on endothelial cells. The increased nerve density associated with vessels strengthened our hypothesis that tissue perfusion is regulated by neural control of vessels and that the interaction between the NGF and VEGF systems is the critical driver for the activated angiogenic process. The interaction of VEGF- and NGF-systems gives new insights into neural control of organ vascularization and perfusion.
引用
收藏
页码:637 / 649
页数:13
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