Isolation and Molecular Characterization of Toxoplasma gondii from Chickens in China

被引：15

作者：

Zhao Guang-wei ^{[1
]}

Shen Bo ^{[1
]}

Xie Qing ^{[1
]}

Xu Li-xin ^{[1
]}

Yan Ruo-feng ^{[1
]}

Song Xiao-kai ^{[1
]}

Adam, Hassan Ibrahim ^{[1
]}

Li Xiang-rui ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China

来源：

JOURNAL OF INTEGRATIVE AGRICULTURE | 2012年 / 11卷 / 08期

关键词：

Toxoplasma gondii; chickens; China; isolation; FREE-RANGE CHICKENS; BIOLOGIC CHARACTERISTICS; UNITED-STATES; SOUTH-AMERICA; STRAINS; INFECTION; BRAZIL;

D O I：

10.1016/S2095-3119(12)60133-6

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

One strain of Toxoplasma gondii was successfully isolated from chickens in China by bioassay in mice. Antibodies and circulating antigens of T. gondii were assayed by the ELISA kits in 100 free range chickens from a rural area surrounding Funing, China. Fifty-three chickens were antibody-positive and 21 chickens were antigen positive. Hearts, brains, spleens, lungs, livers, and kidneys of 21 antibody or antigen-positive chickens were bioassayed in mice. One strain of T. gondii was isolated from 1 of 21 (4.76%) chickens. The isolated T. gondii killed all of the inoculated mice. Genotyping of this isolate using polymorphisms at the loci 5'-SAG2, 3'-SAG2, SAG3, cB21-4, L358, BTUB, and GRA6 revealed that it was Type I. These indicated that it was virulent for mice. This is the first report of isolation of T gondii from chickens in China.

引用

页码：1347 / 1353

页数：7

共 50 条

[21] First Molecular Detection and Genotype Identification of Toxoplasma gondii in Chickens from Farmers' Markets in Fujian Province, Southeastern China [J].

Chu, Meng-Jie ;

Huang, Li-Yuan ;

Miao, Wen-Yuan ;

Song, Ya-Fei ;

Lin, Ying-Sheng ;

Li, Si-Ang ;

Zhou, Dong-Hui .

PATHOGENS, 2023, 12 (10)

[22] Isolation and molecular characterization of Toxoplasma gondii from captive slender-tailed meerkats (Suricata suricatta) with fatal toxoplasmosis in Argentina [J].

Basso, W. ;

More, G. ;

Quiroga, M. A. ;

Pardini, L. ;

Bacigalupe, D. ;

Venturini, L. ;

Valenzuela, M. C. ;

Balducchi, D. ;

Maksimov, P. ;

Schares, G. ;

Venturini, M. C. .

VETERINARY PARASITOLOGY, 2009, 161 (3-4) :201-206

[23] Isolation, genotyping and pathogenicity of a Toxoplasma gondii strain isolated from a Serval (Leptailurus serval) in China [J].

Dong, Hui ;

Su, Ruijing ;

Li, Tongyi ;

Su, Chunlei ;

Zhang, Longxian ;

Yang, Yurong .

TRANSBOUNDARY AND EMERGING DISEASES, 2019, 66 (04) :1796-1802

[24] Pathogenicity of Five Strains of Toxoplasma gondii from Different Animals to Chickens [J].

Wang, Shuai ;

Zhao, Guang-Wei ;

Wang, Wang ;

Zhang, Zhen-Chao ;

Shen, Bo ;

Hassan, I. A. ;

Xie, Qing ;

Yan, Ruo-Feng ;

Song, Xiao-Kai ;

Xu, Li-Xin ;

Li, Xiang-Rui .

KOREAN JOURNAL OF PARASITOLOGY, 2015, 53 (02) :155-162

[25] Genetic Characterization of Toxoplasma gondii from Zoo Wildlife and Pet Birds in Fujian, China [J].

Chen, Renfeng ;

Lin, Xuan ;

Hu, Lingying ;

Chen, Xiaoli ;

Tang, Yao ;

Zhang, Jia ;

Chen, Meizhen ;

Wang, Shoukun ;

Huang, Cuiqin .

IRANIAN JOURNAL OF PARASITOLOGY, 2015, 10 (04) :663-668

[26] Genetic characterization of Toxoplasma gondii isolates from chickens in India by GRA6 gene sequence analysis [J].

Biradar, Shantaveer S. ;

Saravanan, Buddhi C. ;

Tewari, Anup K. ;

Sreekumar, Chirukandoth ;

Sankar, Muthu ;

Sudhakar, Naduvanahalli R. .

ACTA PARASITOLOGICA, 2014, 59 (04) :666-674

[27] Molecular Detection and Genetic Characterization of Toxoplasma gondii from Horses in Three Provinces of China [J].

Ren, Wei-Xin ;

Zhang, Xiao-Xuan ;

Long, Cui-Yu ;

Zhao, Quan ;

Cheng, Tian ;

Ma, Jian-Gang ;

Xu, Peng ;

Hou, Guangyu ;

Ni, Hong-Bo .

VECTOR-BORNE AND ZOONOTIC DISEASES, 2019, 19 (09) :703-707

[28] Molecular detection and genetic characterization of Toxoplasma gondii in pork from Chongqing, southwest China [J].

Zhang, Yehua ;

Xie, Jianhua ;

Mi, Rongsheng ;

Ling, Hongquan ;

Luo, Lu ;

Jia, Haiyan ;

Zhang, Xiaoli ;

Huang, Yan ;

Gong, Haiyan ;

Han, Xiangan ;

Zeng, Zheng ;

Chen, Zhaoguo .

ACTA TROPICA, 2021, 224

[29] Isolation and molecular characterization of Toxoplasma gondii isolated from pigeons and stray cats in Lisbon, Portugal [J].

Vilares, A. ;

Gargate, M. J. ;

Ferreira, I. ;

Martins, S. ;

Julio, C. ;

Waap, H. ;

Angelo, H. ;

Gomes, J. P. .

VETERINARY PARASITOLOGY, 2014, 205 (3-4) :506-511

[30] Toxoplasma gondii prevalence in food animals and rodents in different regions of China: isolation, genotyping and mouse pathogenicity [J].

Wang, Lin ;

Cheng, Hua-Wei ;

Huang, Kai-Quan ;

Xu, Yuan-Hong ;

Li, Yong-Nian ;

Du, Jian ;

Yu, Li ;

Luo, Qing-Li ;

Wei, Wei ;

Jiang, Ling ;

Shen, Ji-Long .

PARASITES & VECTORS, 2013, 6

← 1 2 3 4 5 →