Evaluation of a triplex PCR assay to discriminate Staphylococcus aureus from coagulase-negative staphylococci and determine methicillin resistance from blood cultures

被引：168

作者：

Maes, N ^{[1
]}

Magdalena, J ^{[1
]}

Rottiers, S ^{[1
]}

De Gheldre, Y ^{[1
]}

Struelens, MJ ^{[1
]}

机构：

[1] Free Univ Brussels, Hop Erasme, Microbiol Lab, Microbiol Serv,Ctr Mol Diagnost, B-1070 Brussels, Belgium

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 04期

关键词：

D O I：

10.1128/JCM.40.4.1514-1517.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A triplex PCR targeting the 16S rRNA, mecA, and nuc genes was developed for identification of staphylococci and detection of methicillin resistance. After validation of the assay with a collection of strains of staphylococci and enterococci (n = 169), the assay was evaluated with cultures of blood with gram-positive cocci from 40 patients. Accurate results were obtained for 59 (98%) of 61 cultures within 6 h of growth detection.

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页码：1514 / 1517

页数：4

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