Metabolic and proteomic study of NSO myeloma cell line following the adaptation to protein-free medium

被引：12

作者：

de la Luz-Hernandez, K. R. ^{[1
,4
,5
]}

Rojas-del Calvo, L. ^{[1
]}

Rabasa-Legon, Y. ^{[1
]}

Lage-Castellanos, A. ^{[2
]}

CastilloVitlloch, A. ^{[1
]}

Diaz, J. ^{[3
]}

Gaskell, S. ^{[4
,5
]}

机构：

[1] Ctr Mol Immunol, Res & Dev Direct, Havana, Cuba

[2] Cuban Neurosci Ctr, Havana, Cuba

[3] Univ Havana, Fac Biol, Havana, Cuba

[4] Univ Manchester, Sch Chem, Michael Barber Ctr Mass Spectrometry, Manchester M13 9PL, Lancs, England

[5] Univ Manchester, Manchester Interdisciplinary Bioctr, Manchester M13 9PL, Lancs, England

来源：

JOURNAL OF PROTEOMICS | 2008年 / 71卷 / 02期

关键词：

Metabolomics; Myeloma cell line; Protein-free medium; Quantitative proteomics;

D O I：

10.1016/j.jprot.2008.02.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteomics and metabolomics technologies are potentially useful tool for the study of the very complex process of cell adaptation to protein-free medium. in this work, we used the iTRAQ technology to analyze different protein levels in adapted and non-adapted NSO myeloma cell line. Several proteins with differential expression profile were characterized and quantified. Carbohydrate metabolism, protein synthesis and membrane transport were the principal pathways that change after the adaptation. Changes in lactate production rate with respect to glucose consumption rate were observed according to the changes observed by proteomic. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：133 / 147

页数：15

共 31 条

[1] Swiss-Prot: Juggling between evolution and stability [J].