The DNA-DNA spacing in gemini surfactants-DOPE-DNA complexes

被引：14
作者：
Pullmannova, Petra [1 ]
Funari, Sergio S. [2 ]
Devinsky, Ferdinand [3 ]
Uhrikova, Daniela [1 ]
机构：
[1] Comenius Univ, Fac Pharm, Dept Phys Chem Drugs, SK-83232 Bratislava, Slovakia
[2] DESY, HASYLAB, D-22607 Hamburg, Germany
[3] Comenius Univ, Fac Pharm, Dept Chem Theory Drugs, SK-83232 Bratislava, Slovakia
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2012年 / 1818卷 / 11期
关键词：
DNA; Dioleoylphosphatidylethanolamine; Gemini surfactant; Small angle X-ray diffraction; ALKANEDIYL-ALPHA; OMEGA-BIS(DIMETHYLALKYLAMMONIUM BROMIDE) SURFACTANTS; ELECTRIC CHARGE; PHASE; DELIVERY; BEHAVIOR; EFFICIENCY; VECTORS; PHOSPHATIDYLCHOLINE;
D O I：
10.1016/j.bbamem.2012.05.021
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Gemini surfactants from the homologous series of alkane-alpha,omega-diyl-bis(dodecyldimethylammonium bromide) (CnCS12, number of spacer carbons n = 2 - 12) and dioleoylphosphatidylethanolamine (DOPE) were used for cationic liposome (CL) preparation. CLs condense highly polymerized DNA creating complexes. Small-angle X-ray diffraction identified them as condensed lamellar phase L-alpha(C) in the studied range of molar ratios CnGS12/DOPE in the temperature range 20 - 60 degrees C. The DNA-DNA distance (d(DNA)) is studied in dependence to CnGS12 spacer length and membrane surface charge density. The high membrane surface charge densities (CnGS12/DOPE = 0.35 and 0.4 mol/mol) lead to the linear dependence of d(DNA) vs. n correlating with the interfacial area of the CnGS12 molecule. (c) 2012 Elsevier B.V. All rights reserved.
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收藏
页码：2725 / 2731
页数：7
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