Development of Enzyme-linked Aptamer Assay for Detection of Kanamycin A

The EDC method was employed to conjugate the kanamycin A (KaA) to horseradish peroxidase (HRP). The optimal coating concentration of streptavidin was 8 mg/L and that was determined by chequerboard titration. The optimized assay conditions were investigated by single-factor experiments. An enzyme-linked aptamer assay (ELAA) method was established for the determination of kanamycin A (KaA). The half inhibition concentration (IC50) of the proposed method was 2. 2 mu g/L, the limit of detection (LOD) was 0. 04 mu g/L, and the detection ranged from 0. 07 mu g/L to 71. 5 mu g/L, There were no conspicuous cross reactions with other structural analogues of KaA. The recovery in milk, pork, pork liver, chicken and honey samples was 78%-100% and the RSD was less than 11. 1%. This method is specific and highly sensitive, suitable for the rapid detection of kaA in variable food samples.