Development of Enzyme-linked Aptamer Assay for Detection of Kanamycin A

被引:4
作者
Liu Xin-Jia [1 ]
Jiang Ying-Yan [1 ]
Li Mei-Ying [1 ]
Wang Hong [1 ]
Liu Ying-Ju [2 ]
Lu Lan-Lan [1 ]
Sun Yuan-Ming [1 ]
Lei Hong-Tao [1 ]
机构
[1] Minist Agr, Key Risk Assessment Lab Agr Prod Preservat, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Guangdong, Peoples R China
[2] South China Agr Univ, Coll Sci, Inst Biomat, Guangzhou 510642, Guangdong, Peoples R China
关键词
Single-stranded deoxyribonucleic acid; Aptamer; Kanamycin A; Enzyme linked aptamer assay; ANTIBODY;
D O I
10.3724/SP.J.1096.2013.30018
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The EDC method was employed to conjugate the kanamycin A (KaA) to horseradish peroxidase (HRP). The optimal coating concentration of streptavidin was 8 mg/L and that was determined by chequerboard titration. The optimized assay conditions were investigated by single-factor experiments. An enzyme-linked aptamer assay (ELAA) method was established for the determination of kanamycin A (KaA). The half inhibition concentration (IC50) of the proposed method was 2. 2 mu g/L, the limit of detection (LOD) was 0. 04 mu g/L, and the detection ranged from 0. 07 mu g/L to 71. 5 mu g/L, There were no conspicuous cross reactions with other structural analogues of KaA. The recovery in milk, pork, pork liver, chicken and honey samples was 78%-100% and the RSD was less than 11. 1%. This method is specific and highly sensitive, suitable for the rapid detection of kaA in variable food samples.
引用
收藏
页码:1428 / 1433
页数:6
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