Shape-engineered titanium dioxide nanoparticles (TiO2-NPs): cytotoxicity and genotoxicity in bronchial epithelial cells

被引：60

作者：

Gea, Marta ^{[1
]}

Bonetta, Sara ^{[1
]}

Iannarelli, Luca ^{[2
]}

Giovannozzi, Andrea Mario ^{[2
]}

Maurino, Valter ^{[3
]}

Bonetta, Silvia ^{[1
]}

Hodoroaba, Vasile-Dan ^{[4
]}

Armato, Caterina ^{[1
,5
]}

Rossi, Andrea Mario ^{[2
]}

Schiliro, Tiziana ^{[1
]}

机构：

[1] Univ Turin, Dept Publ Hlth & Pediat, Piazza Polonia 94, I-10126 Turin, Italy

[2] Natl Inst Metrol Res, Qual Life Div, Str Cacce 91, I-10135 Turin, Italy

[3] Univ Turin, Dept Chem, Via Giuria 7, I-10125 Turin, Italy

[4] BAM Fed Inst Mat Res & Testing, Surface Anal & Interfacial Chem Div, D-12200 Berlin, Germany

[5] Italian Inst Technol, Ctr Sustainable Future Technol CSFT PoliTo, Corso Trento 21, I-10129 Turin, Italy

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2019年 / 127卷

关键词：

Shape-engineered TiO2 nanoparticles; Genotoxic and oxidative damage; Comet assay; Cytotoxicity; Raman spectroscopy; IN-VITRO CYTOTOXICITY; OXIDATIVE DNA-DAMAGE; COMET ASSAY; INFLAMMATORY RESPONSE; OXIDE NANOPARTICLES; PARTICLES; TOXICITY; VIVO; NANOSCALE; APOPTOSIS;

D O I：

10.1016/j.fct.2019.02.043

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The aim of this study was to evaluate cytotoxicity (WST-1 assay), LDH release (LDH assay) and genotoxicity (Comet assay) of three engineered TiO2-NPs with different shapes (bipyramids, rods, platelets) in comparison with two commercial TiO2-NPs (P25, food grade). After NPs characterization (SEM/T-SEM and DLS), biological effects of NPs were assessed on BEAS-2B cells in presence/absence of light. The cellular uptake of NPs was analyzed using Raman spectroscopy. The cytotoxic effects were mostly slight. After light exposure, the largest cytotoxicity (WST-1 assay) was observed for rods; P25, bipyramids and platelets showed a similar effect; no effect was induced by food grade. No LDH release was detected, confirming the low effect on plasma membrane. Food grade and platelets induced direct genotoxicity while P25, food grade and platelets caused oxidative DNA damage. No genotoxic or oxidative damage was induced by bipyramids and rods. Biological effects were overall lower in darkness than after light exposure. Considering that only food grade, P25 and platelets (more agglomerated) were internalized by cells, the uptake resulted correlated with genotoxicity. In conclusion, cytotoxicity of NPs was low and affected by shape and light exposure, while genotoxicity was influenced by cellular-uptake and aggregation tendency.

引用

页码：89 / 100

页数：12

共 72 条

[51] Hydroxyl radicals (•OH) are associated with titanium dioxide (TiO2) nanoparticle-induced cytotoxicity and oxidative DNA damage in fish cells [J].