The Effects of Melatonin on Oxidative Stress Parameters and DNA Fragmentation in Testicular Tissue of Rats Exposed to Microwave Radiation

被引:32
作者
Sokolovic, Dusan [1 ]
Djordjevic, Branka [1 ]
Kocic, Gordana [1 ]
Stoimenov, Tatjana J. [1 ]
Stanojkovic, Zoran [2 ]
Sokolovic, Danka M. [2 ]
Veljkvic, Andrei [1 ]
Ristic, Goran [3 ]
Despotovic, Milena [1 ]
Milisavljevic, Dusan [4 ]
Jankovic, Radmilo [5 ]
Binic, Ivana [6 ]
机构
[1] Univ Nis, Fac Med, Dept Biochem, Nish 18000, Serbia
[2] Inst Blood Transfus, Nish, Serbia
[3] Univ Nis, Fac Elect Engn, Nish 18000, Serbia
[4] Univ Nis, Fac Med, Dept Otorhinolaryngol, Nish 18000, Serbia
[5] Univ Nis, Fac Med, Dept Anesthesiol & Intens Care, Nish 18000, Serbia
[6] Nis Clin Ctr, Clin Dermatol & Venereol, Nish, Serbia
来源
ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE | 2015年 / 24卷 / 03期
关键词
melatonin; microwave radiation; testicular tissue; oxidative stress; DNase; LIPID-PEROXIDATION; MOBILE PHONES; DAMAGE; ACID; ASSAY;
D O I
10.17219/acem/43888
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Background. Microwaves from mobile phones are one of the environmental toxicants that are capable of compromising male fertility by inducing oxidative stress and apoptosis in the testes. Melatonin is a lipophilic tryptophan indole amine and a potent antioxidant. Objectives. The aim of the study was to evaluate the effect of melatonin treatment on oxidative stress parameters and DNA fragmentation in the testicular tissue of rats exposed to microwave radiation (4 h/day). Material and Methods. Adult Wistar rats were divided in 4 groups: I - treated with saline; II - treated with melatonin; III - exposed to microwaves; IV - exposed to microwaves and treated with melatonin. The melatonin (2 mg/kg ip) was administered daily. The animals were sacrificed after 20, 40 and 60 days. Results. Melatonin treatment prevented previously registered increases in malondialdehyde after only 20 days. Furthermore, it reversed the effects of microwave exposure on xanthine oxidase (after 40 days) and acid-DNase activity (after 20 days). However, neither protein carbonyl content nor catalase and alkaline Dnase activity were changed due to melatonin treatment. Conclusions. Melatonin exerts potent antioxidant effects in the testes of rats exposed to microwaves by decreasing the intensity of oxidative stress; it also reduces DNA fragmentation
引用
收藏
页码:429 / 436
页数:8
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