Studying macromolecular solutions without wall effects by stroboscopic small-angle x-ray scattering

被引：17

作者：

Graceffa, Rita ^{[1
]}

Burghammer, Manfred ^{[1
]}

Davies, Richard J. ^{[1
]}

Ponchut, Cyril ^{[1
]}

Riekel, Christian ^{[1
]}

机构：

[1] European Synchrotron Radiat Facil, F-38043 Grenoble, France

来源：

APPLIED PHYSICS LETTERS | 2009年 / 94卷 / 06期

关键词：

biological techniques; drops; macromolecules; molecular biophysics; proteins; X-ray scattering; SYNCHROTRON-RADIATION MICRODIFFRACTION; COMPLEX FLUIDS; HYDRATION; STATE;

D O I：

10.1063/1.3078821

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

Small-angle x-ray scattering patterns have been collected stroboscopically from ballistic microdrops of cytochrome C protein solution. The microdrops, measuring around 80 mu m diameter (similar to 268pl), were generated by a drop-on-demand system and traveled at approximately 1.7 m/s through a 3 mu m synchrotron radiation beam. The scattering patterns were accumulated on a pixel detector, which was activated for a few microseconds during the transit time of each microdrop through the microbeam. The stability of the microdrop sequence allowed observing interface scattering from HCl buffer microdrops. The small-angle x-ray scattering data provide information on the protein conformation free of physical boundaries.

引用

页数：3

共 25 条

[1] Conformational landscape of cytochrome c folding studied by microsecond-resolved small-angle x-ray scattering [J].

Akiyama, S ;

Takahashi, S ;

Kimura, T ;

Ishimori, K ;

Morishima, I ;

Nishikawa, Y ;

Fujisawa, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (03) :1329-1334

[2]

BERTHIER J, 2008, MICRODROPS DIGITAL M, P91103

[3]

BRUUS H, 2008, THEORETICAL MICROFLU, P91103

[4] A new batch-processing data-reduction application for X-ray diffraction data [J].

Davies, RJ .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 2006, 39 :267-272

[5] Inkjet printing of polymers: State of the art and future developments [J].

de Gans, BJ ;

Duineveld, PC ;

Schubert, US .

ADVANCED MATERIALS, 2004, 16 (03) :203-213

[6] Visualization of drop-on-demand inkjet: Drop formation and deposition [J].

Dong, Hongming ;

Carr, Wallace W. ;

Morris, Jeffrey F. .

REVIEW OF SCIENTIFIC INSTRUMENTS, 2006, 77 (08)

[7] Rapid prototyping of X-ray microdiffraction compatible continuous microflow foils [J].

Dootz, Rolf ;

Evans, Heather ;

Koester, Sarah ;

Pfohl, Thomas .

SMALL, 2007, 3 (01) :96-100

[8] PRECISE DEPOSITION OF MOLTEN MICRODROPS - THE PHYSICS OF DIGITAL MICROFABRICATION [J].

GAO, FQ ;

SONIN, AA .

PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, 1994, 444 (1922) :533-554

[9]

Glatter O., 1982, Small-angle X-ray scattering", Eds, P119

[10] Synchrotron radiation microdiffraction of ballistic molten wax microdrops [J].

Graceffa, R. ;

Burghammer, M. ;

Davies, R. J. ;

Riekel, C. .

REVIEW OF SCIENTIFIC INSTRUMENTS, 2008, 79 (08)

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