Clarifying Tissue Clearing

被引：833

作者：

Richardson, Douglas S. ^{[1
,2
]}

Lichtman, Jeff W. ^{[1
,2
,3
]}

机构：

[1] Harvard Univ, Harvard Ctr Biol Imaging, Cambridge, MA 02138 USA

[2] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

[3] Harvard Univ, Ctr Brain Sci, Cambridge, MA 02138 USA

来源：

CELL | 2015年 / 162卷 / 02期

关键词：

SINGLE-CELL RESOLUTION; BACKGROUND AUTOFLUORESCENCE; FLUORESCENCE MICROSCOPY; BRAIN; PLANAR; REDUCTION; PROTEINS; AGENT;

D O I：

10.1016/j.cell.2015.06.067

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Biological specimens are intrinsically three dimensional; however, because of the obscuring effects of light scatter, imaging deep into a tissue volume is problematic. Although efforts to eliminate the scatter by "clearing'' the tissue have been ongoing for over a century, there have been a large number of recent innovations. This Review introduces the physical basis for light scatter in tissue, describes the mechanisms underlying various clearing techniques, and discusses several of the major advances in light microscopy for imaging cleared tissue.

引用

页码：246 / 257

页数：12

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