Association between Polymorphisms in Interleukin-17A and-17F Genes and Chronic Periodontal Disease

被引:36
作者
Correa, Joice Dias [1 ]
Moreira Madeira, Mila Fernandes [2 ]
Resende, Renata Goncalves [1 ]
Correia-Silva, Jeane de Fatima [1 ]
Gomez, Ricardo Santiago [1 ]
de Souza, Danielle da Gloria [2 ]
Teixeira, Mauro Martins [3 ]
Queiroz-Junior, Celso Martins [1 ]
da Silva, Tarcilia Aparecida [1 ]
机构
[1] Univ Fed Minas Gerais, Fac Odontol, Dept Clin Patol & Cirurgia Odontol, BR-31270901 Belo Horizonte, MG, Brazil
[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Microbiol, BR-31270901 Belo Horizonte, MG, Brazil
[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, BR-31270901 Belo Horizonte, MG, Brazil
关键词
GINGIVAL CREVICULAR FLUID; NECROSIS-FACTOR-ALPHA; GROWTH-FACTOR-BETA; GAMMA GENE; BONE LOSS; SUSCEPTIBILITY; CYTOKINES; CELLS; IL-17; EXPRESSION;
D O I
10.1155/2012/846052
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Objective. Interleukin-17 (IL-17) is a cytokine that induces neutrophil recruitment and the release of inflammatory mediators in several inflammatory conditions; nevertheless, the involvement of IL-17 gene polymorphisms in chronic periodontitis (CP) has not been addressed yet. Our aim was to evaluate the association between periodontal status and the polymorphisms IL-17A G197A and IL-17F C7488T in subjects with CP along with their impact on levels of inflammatory mediators. Material and Methods. Genomic DNA was obtained from 30 CP patients and 30 healthy controls (HCs). IL-17A G197A and IL-17F C7488T polymorphisms were determined using PCR-RFLP. Serum and periodontal tissues were collected and processed for ELISA, myeloperoxidase (MPO), and/or microscopic analysis. Results. The frequencies of genotypes in the CP group were significantly different from those of HC. Odds ratio indicated that increased risks for CP were associated with the -197A allele, not with the -7488T allele. In addition, the -197A allele was correlated with worse clinical parameters, higher MPO activity, and increased expression of inflammatory mediators (IL-17A and IL-8) than the other genotypes. Conclusions. These results indicate that the IL-17A -197A allele is associated with increased risk for CP, likely because this genotype relates to the enhanced inflammation in periodontal tissues.
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页数:9
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