Gene delivery to cells on a miniaturized multiwell plate for high-throughput gene function analysis

被引：0

作者：

Njatawidjaja, Ellyana ^{[1
]}

Iwata, Hiroo ^{[1
]}

机构：

[1] Kyoto Univ, Dept Reparat Mat, Inst Frontier Med Sci, Sakyo Ku, Kyoto 6068507, Japan

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2008年 / 392卷 / 03期

关键词：

miniaturized multiwell plate; transfectional microarray; electroporation; plasmid DNA; gene delivery;

D O I：

10.1007/s00216-008-2281-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The transfectional microarray is a promising tool to conduct high-throughput analysis of gene function. In this study, a miniaturized multiwell plate was prepared by applying a silicone rubber sheet with holes on a gold electrode. The combination of electroporation and a miniaturized multiwell plate successfully achieved spatially controlled gene delivery with absence of cross-contamination between genes. Furthermore, we found that gene delivery efficiency was not dependent on conditions such as plasmid loading, electric field strength, and pulse duration.

引用

页码：405 / 408

页数：4

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