Gene delivery to cells on a miniaturized multiwell plate for high-throughput gene function analysis

被引:0
|
作者
Njatawidjaja, Ellyana [1 ]
Iwata, Hiroo [1 ]
机构
[1] Kyoto Univ, Dept Reparat Mat, Inst Frontier Med Sci, Sakyo Ku, Kyoto 6068507, Japan
关键词
miniaturized multiwell plate; transfectional microarray; electroporation; plasmid DNA; gene delivery;
D O I
10.1007/s00216-008-2281-8
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The transfectional microarray is a promising tool to conduct high-throughput analysis of gene function. In this study, a miniaturized multiwell plate was prepared by applying a silicone rubber sheet with holes on a gold electrode. The combination of electroporation and a miniaturized multiwell plate successfully achieved spatially controlled gene delivery with absence of cross-contamination between genes. Furthermore, we found that gene delivery efficiency was not dependent on conditions such as plasmid loading, electric field strength, and pulse duration.
引用
收藏
页码:405 / 408
页数:4
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