Is Real-Time PCR Better than Conventional PCR for Mycobacterium tuberculosis Complex Detection in Clinical Samples?

被引：25

作者：

Tortoli, Enrico ^{[1
]}

Urbano, Pasquale ^{[2
]}

Marcelli, Fiorella ^{[3
]}

Simonetti, Tullia M. ^{[3
]}

Cirillo, Daniela M. ^{[1
]}

机构：

[1] Ist Sci San Raffaele, Emerging Bacterial Pathogens Unit, I-20132 Milan, Italy

[2] Univ Florence, Inst Microbiol, Florence, Italy

[3] Careggi Univ Hosp, Reg Reference Ctr Mycobacteria, Florence, Italy

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 08期

关键词：

COBAS AMPLICOR MTB; RESPIRATORY SPECIMENS; RAPID DETECTION; NONRESPIRATORY SPECIMENS; PULMONARY TUBERCULOSIS; DIAGNOSIS; SYSTEM; ASSAY; AMPLIFICATION; SMEAR;

D O I：

10.1128/JCM.01412-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Cobas Amplicor MTB and later Cobas TaqMan MTB were used to test a very large series of consecutive specimens received for tuberculosis diagnosis. Performance parameters were estimated and compared overall and for separate specimen categories. Both systems showed excellent specificity, and that of TaqMan was the higher. The sensitivities were similar but satisfactory only with respiratory specimens and smear-positive samples.

引用

页码：2810 / 2813

页数：4

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