The genetic dependence of RecBCD-Gam mediated double strand end repair in Escherichia coli

被引：14

作者：

Paskvan, I ^{[1
]}

Salaj-Smic, E ^{[1
]}

Ivancic-Bace, I ^{[1
]}

Zahradka, K ^{[1
]}

Trgovcevic, Z ^{[1
]}

Brcic-Kostic, K ^{[1
]}

机构：

[1] Rudjer Boskovic Inst, Dept Mol Genet, Zagreb 10002, Croatia

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 205卷 / 02期

关键词：

phage lambda; RecBCD enzyme; lambda Gam protein; recombination; repair; Escherichia coli;

D O I：

10.1016/S0378-1097(01)00478-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The repair of double strand breaks after gamma -irradiation in wild-type Escherichia coli lysogenic for lambda cI857 red3 is more efficient when lambda Gain protein is present. This phenomenon, called gain dependent radioresistance, requires the interaction of RecBCD enzyme and Gam protein. We compared cell survival after gamma -irradiation in wild-type and mutant lysogens with and without induction of Gam. by transient heat treatment of the cells (6 min, 42 degreesC). The main conclusions are: (1) the RecBCD-Gam pathway of recombination repair is similar but not equivalent to RecBCD double dagger, a pathway operating in recD mutants; (2) the RecBCD-Gam pathway is dependent on recJ, recQ and recN gene products and it is proposed that the RecBCD-Gam complex has ability to load RecA protein onto single strand DNA. (C) 2001 Federation of European Micro biological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：299 / 303

页数：5

共 23 条

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