Ionic Liquids as Mobile Phase Additives for Feasible Assay of Naphazoline in Pharmaceutical Formulation by HPTLC-UV-Densitometric Method

被引：23

作者：

Marszall, Michal Piotr ^{[1
]}

Sroka, Wiktor Dariusz ^{[1
]}

Balinowska, Aleksandra ^{[1
]}

Mieszkowski, Dominik ^{[1
]}

Koba, Marcin ^{[2
]}

Kaliszan, Roman ^{[3
]}

机构：

[1] Nicolaus Copernicus Univ, Dept Med Chem, Coll Med Bydgoszcz, PL-85094 Bydgoszcz, Poland

[2] Nicolaus Copernicus Univ, Dept Toxicol, Coll Med Bydgoszcz, PL-85094 Bydgoszcz, Poland

[3] Med Univ Gdansk, Dept Biopharmaceut & Pharmacodynam, PL-80416 Gdansk, Poland

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2013年 / 51卷 / 06期

关键词：

ROOM-TEMPERATURE; HPLC; PERFORMANCE; SEPARATION; CHROMATOGRAPHY; SYSTEMS;

D O I：

10.1093/chromsci/bms168

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A specific and reliable high-performance thin layer chromatography method with densitometry detection has been developed for the determination of naphazoline nitrate in nasal drops. The best separation of the basic analyte, without spot tailing, was achieved by using a mobile phase composed of acetonitrile-water (60:40, v/v), adding 1.5 % (v/v) imidazolium-class ionic liquid and covering the plates with a stationary phase based on RP-18 with F-254S (10 x 20 cm). The presented results confirm that imidazolium tetrafluoroborate ionic liquids are efficient suppressors of free silanols, which are considered to be responsible for troublesome and irreproducible chromatographic determinations of basic compounds. The developed chromatographic system was found to be convenient in use and to provide a repeatable assay of naphazoline nitrate in nasal drops, which could not be obtained with the use of standard silanol suppressing mobile phase additives such as triethylamine or dimethyloctylamine.

引用

页码：560 / 565

页数：6

共 26 条

[1] DIRECT DETERMINATION OF BENZALKONIUM CHLORIDE IN OPHTHALMIC SYSTEMS BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

AMBRUS, G ;

TAKAHASHI, LT ;

MARTY, PA .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1987, 76 (02) :174-176

[2]

Antoniou CG, 2011, J AOAC INT, V94, P703

[3] Behavior of peptides and computer-assisted optimization of peptides separations in a normal-phase thin-layer chromatography system with and without the addition of ionic liquid in the eluent [J].

Baczek, T ;

Marszall, MP ;

Kaliszan, R ;

Walijewski, L ;

Makowiecka, W ;

Sparzak, B ;

Grzonka, Z ;

Wisniewska, K ;

Juszczyk, P .

BIOMEDICAL CHROMATOGRAPHY, 2005, 19 (01) :1-8

[4] Ionic liquids in separation techniques [J].

Berthod, A. ;

Ruiz-Angel, Mt ;

Carda-Broch, S. .

JOURNAL OF CHROMATOGRAPHY A, 2008, 1184 (1-2) :6-18

[5] Nonmolecular solvents in separation methods: Dual nature of room temperature ionic liquids [J].

Berthod, A ;

Ruiz-Angel, MJ ;

Huguet, S .

ANALYTICAL CHEMISTRY, 2005, 77 (13) :4071-4080

[6] A review of ionic liquids in chromatographic and electromigration techniques [J].

Buszewski, Boguslaw ;

Studzinska, Sylwia .

CHROMATOGRAPHIA, 2008, 68 (1-2) :1-10

[7] Trends and progress in the characterization of stationary phases for reversed-phase liquid chromatography [J].

Claessens, HA .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2001, 20 (10) :563-583

[8] Silanol suppressing potency of alkyl-imidazolium ionic liquids on C18 stationary phases [J].

Fernandez-Navarro, J. J. ;

Torres-Lapasio, J. R. ;

Ruiz-Angel, M. J. ;

Garcia-Alvarez-Coque, M. C. .

JOURNAL OF CHROMATOGRAPHY A, 2012, 1232 :166-175

[9] The role of the dual nature of ionic liquids in the reversed-phase liquid chromatographic separation of basic drugs [J].

Fernandez-Navarro, J. J. ;

Garcia-Alvarez-Coque, M. C. ;

Ruiz-Angel, M. J. .

JOURNAL OF CHROMATOGRAPHY A, 2011, 1218 (03) :398-407

[10] The performance of 1-ethyl-3-methylimidazolium tetrafluoroborate ionic liquid as mobile phase additive in HPLC-based lipophilicity assessment [J].

Giaginis, Costas ;

Tsantili-Kakoulidou, Anna .

BIOMEDICAL CHROMATOGRAPHY, 2011, 25 (05) :606-612

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