An Improved Micro Propagation System for Successful Transformation of Cauliflower (Brassica oleracea var. botrytis)

被引：0

作者：

Al-Swedi, F. G. ^{[1
]}

Lane, S. ^{[1
]}

Fuller, M. P. ^{[1
]}

机构：

[1] Univ Plymouth, Fac Sci & Technol, Sch Biomed & Biol Sci, Plymouth PL4 8AA, Devon, England

来源：

VII INTERNATIONAL SYMPOSIUM ON IN VITRO CULTURE AND HORTICULTURAL BREEDING | 2012年 / 961卷

关键词：

cauliflower; micro propagation; Agrobacterium tumefaciens; TUMEFACIENS-MEDIATED TRANSFORMATION; AGROBACTERIUM-TUMEFACIENS; REGENERATION; EXPRESSION; PROTOCOL; GROWTH; GENES; NAPUS;

D O I：

10.17660/ActaHortic.2012.961.19

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Cauliflower is described as a recalcitrant plant to the genetic transformation process from Agrobacterium. Cauliflower curd meristems were used in a fractionation and sieving protocol to produce microshoots in in vitro culture. This technique produced many thousands of small explants suitable for transfection studies. Explants were successfully transformed following standard transfection with A. tumefaciens carrying a construct with Kanamycin(R), GUS and the Ascorbate Peroxidase (APX) gene. Selection using Kanamycin produced normal plant phenotypic plantlets and these putative transformants were confirmed using GUS staining and PCR of the APX gene sequence. The transfection conditions were optimised during this study and 105 Agrobacterium cells/5 plants/pot successfully produced 1 transformed plant/pot.

引用

页码：167 / 174

页数：8

共 27 条

[1]

Anderson J.A.W., 1977, HORTIC SCI, V102, P67

[2]

[Anonymous], 1987, 707 MAFF, P25

[3]

BECHTOLD N, 1993, CR ACAD SCI III-VIE, V316, P1194

[4] In vitro propagation of cauliflower, Brassica oleracea var. botrytis for hybrid seed production [J].

Bhalla, PL ;

de Weerd, N .

PLANT CELL TISSUE AND ORGAN CULTURE, 1999, 56 (02) :89-95

[5] Agrobacterium tumefaciens-mediated transformation of cauliflower, Brassica oleracea var. botrytis [J].

Bhalla, PL ;

Smith, N .

MOLECULAR BREEDING, 1998, 4 (06) :531-541

[6] Agrobacterium-mediated transformation of cauliflower:: optimization of protocol and development of Bt-transgenic cauliflower [J].

Chakrabarty, R ;

Viswakarma, N ;

Bhat, SR ;

Kirti, PB ;

Singh, BD ;

Chopra, VL .

JOURNAL OF BIOSCIENCES, 2002, 27 (05) :495-502

[7]

CHANGHE Y, 2002, PLANT CELL TISS ORG, V71, P147

[8] Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].

Clough, SJ ;

Bent, AF .

PLANT JOURNAL, 1998, 16 (06) :735-743

[9] TRANSFORMATION OF BRASSICA-NAPUS AND BRASSICA-OLERACEA USING AGROBACTERIUM-TUMEFACIENS AND THE EXPRESSION OF THE BAR AND NEO GENES IN THE TRANSGENIC PLANTS [J].

DEBLOCK, M ;

DEBROUWER, D ;

TENNING, P .

PLANT PHYSIOLOGY, 1989, 91 (02) :694-701

[10] TRANSFORMATION OF CAULIFLOWER (BRASSICA-OLERACEA L VAR BOTRYTIS) - AN EXPERIMENTAL SURVEY [J].

EIMERT, K ;

SIEGEMUND, F .

PLANT MOLECULAR BIOLOGY, 1992, 19 (03) :485-490

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