In this paper, well-ordered gold nanowires were synthesized by utilizing anodic aluminium oxide (AAO) templates in combination with direct electrodeposition. The morphology and structure of AAO template and gold nanowires were characterized by scanning electron microscope (SEM), transmission electron microscope (TEM) and X-ray diffraction, respectively. Glucose biosensors were fabricated by chemical cross linking method with glutaraldehyde in the absence and presence of gold nanowires. Cyclic voltammogram and amperometric mode were used to measure the performance of glucose biosensors. Lower detection limit of 5 mu M and wide linear range between 20 mu M and 2 mM with fast response were achieved by glucose biosensor in the presence of gold nanowires. (c) 2012 Elsevier B.V. All rights reserved.