Evaluation of a recombinant measles virus expressing hepatitis C virus envelope proteins by infection of human PBL-NOD/Scid/Jak3null mouse

被引：8

作者：

Satoh, Masaaki ^{[1
]}

Saito, Makoto ^{[1
]}

Tanaka, Kohsuke ^{[1
]}

Iwanaga, Sumako ^{[2
]}

Ali, Salem Nagla Elwy Salem ^{[1
,4
]}

Seki, Takahiro ^{[5
]}

Okada, Seiji ^{[2
]}

Kohara, Michinori ^{[3
]}

Harada, Shinji ^{[4
]}

Kai, Chieko ^{[5
]}

Tsukiyama-Kohara, Kyoko ^{[1
]}

机构：

[1] Kumamoto Univ, Fac Life Sci, Dept Expt Phylaxiol, Kumamoto, Kumamoto 8608556, Japan

[2] Kumamoto Univ, Ctr AIDS Res, Div Hematopoiesis, Kumamoto, Kumamoto 8608556, Japan

[3] Tokyo Metropolitan Inst Med Sci, Dept Microbiol & Cell Biol, Tokyo, Japan

[4] Kumamoto Univ, Fac Life Sci, Dept Med Virol, Kumamoto, Kumamoto 8608556, Japan

[5] Univ Tokyo, Inst Med Sci, Lab Anim Res Ctr, Tokyo 1138654, Japan

来源：

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2010年 / 33卷 / 06期

关键词：

MV; HCV; E1; E2; Human PBL; NOD/Scid/Jak3null mouse;

D O I：

10.1016/j.cimid.2010.02.006

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In this study, we infected NOD/Scid/Jak3null mice engrafted human peripheral blood leukocytes (hu-PBL-NOJ) with measles virus Edmonston B strain (MV-Edm) expressing hepatitis C virus (HCV) envelope proteins (rMV-E1E2) to evaluate the immunogenicity as a vaccine candidate. Although human leukocytes could be isolated from the spleen of mock-infected mice during the 2-weeks experiment, the proportion of engrafted human leukocytes in mice infected with MV (10(3)-10(5) pfu) or rMV-E1E2 (10(4) pfu) was decreased. Viral infection of the splenocytes was confirmed by the development of cytopathic effects (CPEs) in co-cultures of splenocytes and B95a cells and verified using RT-PCR. Finally, human antibodies againstMVwere more frequently observed than E2-specific antibodies in serum from mice infected with a low dose of virus (MV, 10(0)-10(1) pfu, and rMV-E1E2, 10(1)-10(2) pfu). These results showed the possibility of hu-PBL-NOJ mice for the evaluation of the immunogenicity of viral proteins. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：E81 / E88

页数：8

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