Mass spectrometry for the analysis of highly charged sulfated carbohydrates

被引：46

作者：

Chi, LL

Amster, J

Linhardt, RJ ^{[1
]}

机构：

[1] Rensselaer Polytech Inst, Dept Chem & Biol Chem, Troy, NY 12180 USA

[2] Rensselaer Polytech Inst, Dept Biol & Chem Engn, Troy, NY 12180 USA

[3] Rensselaer Polytech Inst, Dept Biol, Troy, NY 12180 USA

[4] Univ Georgia, Dept Chem, Athens, GA 30602 USA

来源：

CURRENT ANALYTICAL CHEMISTRY | 2005年 / 1卷 / 03期

关键词：

mass spectrometry; sulfated carbohydrates; glycosaminoglycan; oligosaccharide; structure; sequence;

D O I：

10.2174/157341105774573929

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Sulfated polysaccharides and their oligosaccharide components play an important biological role in physiological and pathophysiological processes. Glycosaminoglycans, such as heparin, are currently used as pharmaceutical agents to control coagulation. Moreover, there are intensive efforts in developing rapid approaches for the analysis of such bioactive carbohydrates to assess their pharmacological activity and in glycomic applications. Soft ionization methods of mass spectrometry have been applied with recent success in the analysis of sulfated carbohydrates. This review will examine the application of mass spectrometry in the compositional analysis and structural characterization of these important highly charged molecules.

引用

页码：223 / 240

页数：18

共 92 条

[1] Characterization of a Bacteroides species from human intestine that degrades glycosaminoglycans [J].