Quantitative imaging of complex samples by spiral phase contrast microscopy

被引:254
作者
Bernet, S [1 ]
Jesacher, A [1 ]
Fürhapter, S [1 ]
Maurer, C [1 ]
Ritsch-Marte, M [1 ]
机构
[1] Innsbruck Med Univ, Div Biomed Phys, A-6020 Innsbruck, Austria
关键词
D O I
10.1364/OE.14.003792
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Recently a spatial spiral phase filter in a Fourier plane of a microscopic imaging setup has been demonstrated to produce edge enhancement and relief-like shadow formation of amplitude and phase samples. Here we demonstrate that a sequence of at least 3 spatially filtered images, which are recorded with different rotational orientations of the spiral phase plate, can be used to obtain a quantitative reconstruction of both, amplitude and phase information of a complex microscopic sample, i.e. an object consisting of mixed absorptive and refractive components. The method is demonstrated using a calibrated phase sample, and an epithelial cheek cell. (c) 2006 Optical Society of America.
引用
收藏
页码:3792 / 3805
页数:14
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