Quantitative imaging of complex samples by spiral phase contrast microscopy

被引：250

作者：

Bernet, S ^{[1
]}

Jesacher, A ^{[1
]}

Fürhapter, S ^{[1
]}

Maurer, C ^{[1
]}

Ritsch-Marte, M ^{[1
]}

机构：

[1] Innsbruck Med Univ, Div Biomed Phys, A-6020 Innsbruck, Austria

来源：

OPTICS EXPRESS | 2006年 / 14卷 / 09期

关键词：

D O I：

10.1364/OE.14.003792

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Recently a spatial spiral phase filter in a Fourier plane of a microscopic imaging setup has been demonstrated to produce edge enhancement and relief-like shadow formation of amplitude and phase samples. Here we demonstrate that a sequence of at least 3 spatially filtered images, which are recorded with different rotational orientations of the spiral phase plate, can be used to obtain a quantitative reconstruction of both, amplitude and phase information of a complex microscopic sample, i.e. an object consisting of mixed absorptive and refractive components. The method is demonstrated using a calibrated phase sample, and an epithelial cheek cell. (c) 2006 Optical Society of America.

引用

页码：3792 / 3805

页数：14

共 20 条

[1] Linear phase imaging using differential interference contrast microscopy
Arnison, MR
Larkin, KG
Sheppard, CJR
Smith, NI
Cogswell, CJ
[J]. JOURNAL OF MICROSCOPY-OXFORD, 2004, 214 (01): : 7 - 12
[2] Optical processing with vortex-producing lenses
Crabtree, K
Davis, JA
Moreno, I
[J]. APPLIED OPTICS, 2004, 43 (06) : 1360 - 1367
[3] Image processing with the radial Hilbert transform: theory and experiments
Davis, JA
McNamara, DE
Cottrell, DM
Campos, J
[J]. OPTICS LETTERS, 2000, 25 (02) : 99 - 101
[4] Fürhapter S, 2005, OPT LETT, V30, P1953, DOI 10.1364/OL.30.001953
[5] Spiral phase contrast imaging in microscopy
Fürhapter, S
Jesacher, A
Bernet, S
Ritsch-Marte, M
[J]. OPTICS EXPRESS, 2005, 13 (03): : 689 - 694
[6] GENERATION OF OPTICAL-PHASE SINGULARITIES BY COMPUTER-GENERATED HOLOGRAMS
HECKENBERG, NR
MCDUFF, R
SMITH, CP
WHITE, AG
[J]. OPTICS LETTERS, 1992, 17 (03) : 221 - 223
[7] ZONE PLATES PERFORMING GENERALIZED HANKEL-TRANSFORMS AND THEIR METROLOGICAL APPLICATIONS
JAROSZEWICZ, Z
KOLODZIEJCZYK, A
[J]. OPTICS COMMUNICATIONS, 1993, 102 (5-6) : 391 - 396
[8] Shadow effects in spiral phase contrast microscopy -: art. no. 233902
Jesacher, A
Fürhapter, S
Bernet, S
Ritsch-Marte, M
[J]. PHYSICAL REVIEW LETTERS, 2005, 94 (23)
[9] JESACHER A, 2006, IN PRESS JOSA A
[10] PHASE-SHIFTING COMMON-PATH INTERFEROMETER USING A LIQUID-CRYSTAL PHASE MODULATOR
KADONO, H
OGUSU, M
TOYOOKA, S
[J]. OPTICS COMMUNICATIONS, 1994, 110 (3-4) : 391 - 400

← 1 2 →