Quantitative imaging of complex samples by spiral phase contrast microscopy

被引：254

作者：

Bernet, S ^{[1
]}

Jesacher, A ^{[1
]}

Fürhapter, S ^{[1
]}

Maurer, C ^{[1
]}

Ritsch-Marte, M ^{[1
]}

机构：

[1] Innsbruck Med Univ, Div Biomed Phys, A-6020 Innsbruck, Austria

来源：

OPTICS EXPRESS | 2006年 / 14卷 / 09期

关键词：

D O I：

10.1364/OE.14.003792

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Recently a spatial spiral phase filter in a Fourier plane of a microscopic imaging setup has been demonstrated to produce edge enhancement and relief-like shadow formation of amplitude and phase samples. Here we demonstrate that a sequence of at least 3 spatially filtered images, which are recorded with different rotational orientations of the spiral phase plate, can be used to obtain a quantitative reconstruction of both, amplitude and phase information of a complex microscopic sample, i.e. an object consisting of mixed absorptive and refractive components. The method is demonstrated using a calibrated phase sample, and an epithelial cheek cell. (c) 2006 Optical Society of America.

引用

页码：3792 / 3805

页数：14

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