Creation of an HDAC-Based Yeast Screening Method for Evaluation of Marine-Derived Actinomycetes: Discovery of Streptosetin A

被引：16

作者：

Amagata, Taro ^{[1
]}

Xiao, Jing ^{[1
]}

Chen, Yi-Pei ^{[1
]}

Holsopple, Nicholas ^{[1
]}

Oliver, Allen G. ^{[2
]}

Gokey, Trevor ^{[1
]}

Guliaev, Anton B. ^{[1
]}

Minoura, Katsuhiko ^{[3
]}

机构：

[1] San Francisco State Univ, Dept Chem & Biochem, San Francisco, CA 94132 USA

[2] Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA

[3] Osaka Univ Pharmaceut Sci, Takatsuki, Osaka 5691094, Japan

来源：

JOURNAL OF NATURAL PRODUCTS | 2012年 / 75卷 / 12期

关键词：

ELECTRONIC CIRCULAR-DICHROISM; ABSOLUTE-CONFIGURATION; INHIBITORS; SIRT2; IDENTIFICATION; BIOSYNTHESIS; METABOLITE; EQUISETIN; FAMILY; CANCER;

D O I：

10.1021/np300640g

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A histone deacetylase (HDAC)-based yeast assay employing a URA3 reporter gene was applied as a primary screen to evaluate a marine-derived actinomycete extract library and identify human class III HDAC (SIRT) inhibitors. On the basis of the bioassay-guided purification, a new compound designated as streptosetin A (1) was obtained from one of the active strains identified through the yeast assay. The gross structure of the new compound was elucidated from the ID and 2D NMR data. The absolute stereostructure of 1 was determined based on X-ray crystal structure analysis and simulation of ECD spectra using time-dependent density functional theory calculations. This compound showed weak inhibitory activity against yeast Sir2p and human SIRT1 and SIRT2.

引用

页码：2193 / 2199

页数：7

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