Modulation of the current signatures of DNA abasic site adducts in the α-hemolysin ion channel

被引：11

作者：

An, Na ^{[1
]}

White, Henry S. ^{[1
]}

Burrows, Cynthia J. ^{[1
]}

机构：

[1] Univ Utah, Dept Chem, Salt Lake City, UT 84112 USA

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 93期

关键词：

SINGLE-STRANDED-DNA; POLYNUCLEOTIDE MOLECULES; BIOLOGICAL NANOPORE; DISCRIMINATION; TRANSLOCATION; RNA;

D O I：

10.1039/c2cc36366f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Electrical current signatures of DNA adducts were investigated during immobilization of strands inside the membrane-bound alpha-hemolysin ion channel. The current blockages produced by these adducts were found to depend on both size and shape, providing insights into the DNA-protein interactions and the size limitation of bulky adducts to be translocated.

引用

收藏

页码：11410 / 11412

页数：3

相关论文

共 23 条

[1] Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules [J].

Akeson, M ;

Branton, D ;

Kasianowicz, JJ ;

Brandin, E ;

Deamer, DW .

BIOPHYSICAL JOURNAL, 1999, 77 (06) :3227-3233

[2] Crown ether-electrolyte interactions permit nanopore detection of individual DNA abasic sites in single molecules [J].

An, Na ;

Fleming, Aaron M. ;

White, Henry S. ;

Burrows, Cynthia J. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (29) :11504-11509

[3] Molecular bases of cyclodextrin adapter interactions with engineered protein nanopores [J].

Banerjee, Arijit ;

Mikhailova, Ellina ;

Cheley, Stephen ;

Gu, Li-Qun ;

Montoya, Michelle ;

Nagaoka, Yasuo ;

Gouaux, Eric ;

Bayley, Hagan .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (18) :8165-8170

[4] The potential and challenges of nanopore sequencing [J].

Branton, Daniel ;

Deamer, David W. ;

Marziali, Andre ;

Bayley, Hagan ;

Benner, Steven A. ;

Butler, Thomas ;

Di Ventra, Massimiliano ;

Garaj, Slaven ;

Hibbs, Andrew ;

Huang, Xiaohua ;

Jovanovich, Stevan B. ;

Krstic, Predrag S. ;

Lindsay, Stuart ;

Ling, Xinsheng Sean ;

Mastrangelo, Carlos H. ;

Meller, Amit ;

Oliver, John S. ;

Pershin, Yuriy V. ;

Ramsey, J. Michael ;

Riehn, Robert ;

Soni, Gautam V. ;

Tabard-Cossa, Vincent ;

Wanunu, Meni ;

Wiggin, Matthew ;

Schloss, Jeffery A. .

NATURE BIOTECHNOLOGY, 2008, 26 (10) :1146-1153

[5] Automated forward and reverse ratcheting of DNA in a nanopore at 5-Å precision [J].

Cherf, Gerald M. ;

Lieberman, Kate R. ;

Rashid, Hytham ;

Lam, Christopher E. ;

Karplus, Kevin ;

Akeson, Mark .

NATURE BIOTECHNOLOGY, 2012, 30 (04) :344-348

[6]

Clarke J, 2009, NAT NANOTECHNOL, V4, P265, DOI [10.1038/NNANO.2009.12, 10.1038/nnano.2009.12]

[7] Characterization of nucleic acids by nanopore analysis [J].

Deamer, DW ;

Branton, D .

ACCOUNTS OF CHEMICAL RESEARCH, 2002, 35 (10) :817-825

[8] Nucleobase recognition at alkaline pH and apparent pKa of single DNA bases immobilised within a biological nanopore [J].

Franceschini, Lorenzo ;

Mikhailova, Ellina ;

Bayley, Hagan ;

Maglia, Giovanni .

CHEMICAL COMMUNICATIONS, 2012, 48 (10) :1520-1522

[9] THERMODYNAMIC AND KINETIC DATA FOR CATION MACROCYCLE INTERACTION [J].

IZATT, RM ;

BRADSHAW, JS ;

NIELSEN, SA ;

LAMB, JD ;

CHRISTENSEN, JJ .

CHEMICAL REVIEWS, 1985, 85 (04) :271-339

[10] Urea Facilitates the Translocation of Single-Stranded DNA and RNA Through the α-Hemolysin Nanopore [J].

Japrung, Deanpen ;

Henricus, Marsiyana ;

Li, Qiuhong ;

Maglia, Giovanni ;

Bayley, Hagan .

BIOPHYSICAL JOURNAL, 2010, 98 (09) :1856-1863