Using FRET to measure the time it takes for a cell to destroy a virus

被引：6
作者：
Benjamin, Candace E. [1 ]
Chen, Zhuo [1 ]
Brohlin, Olivia R. [1 ]
Lee, Hamilton [1 ]
Shahrivarkevishahi, Arezoo [1 ]
Boyd, Stefanie [2 ]
Winkler, Duane D. [2 ]
Gassensmith, Jeremiah J. [1 ,3 ]
机构：
[1] Univ Texas Dallas, Dept Chem & Biochem, Richardson, TX 75080 USA
[2] Univ Texas Dallas, Dept Biol Sci, Richardson, TX 75080 USA
[3] Univ Texas Dallas, Dept Bioengn, Richardson, TX 75080 USA
来源：
NANOSCALE | 2020年 / 12卷 / 16期
基金：
美国国家科学基金会;
关键词：
BACTERIOPHAGE Q-BETA; RATIONAL DESIGN; PARTICLES; PLATFORM; FUNCTIONALIZATION; NANOPARTICLES; RESPONSES; DELIVERY;
D O I：
10.1039/c9nr09816j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The emergence of viral nanotechnology over the preceding two decades has created a number of intellectually captivating possible translational applications; however, thein vitrofate of the viral nanoparticles in cells remains an open question. Herein, we investigate the stability and lifetime of virus-like particle (VLP) Q beta-a representative and popular VLP for several applications-following cellular uptake. By exploiting the available functional handles on the viral surface, we have orthogonally installed the known FRET pair, FITC and Rhodamine B, to gain insight of the particle's behaviorin vitro. Based on these data, we believe VLPs undergo aggregation in addition to the anticipated proteolysis within a few hours of cellular uptake.
引用
收藏
页码：9124 / 9132
页数：9
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  • [1] Site-Selective Nucleation and Size Control of Gold Nanoparticle Photothermal Antennae on the Pore Structures of a Virus
    Benjamin, Candace E.
    Chen, Zhuo
    Kang, Peiyuan
    Wilson, Blake A.
    Li, Na
    Nielsen, Steven O.
    Qin, Zhenpeng
    Gassensmith, Jeremiah J.
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2018, 140 (49) : 17226 - 17233
  • [2] Creating highly amplified enzyme-linked immunosorbent assay signals from genetically engineered bacteriophage
    Brasino, Michael
    Lee, Ju Hun
    Cha, Jennifer N.
    [J]. ANALYTICAL BIOCHEMISTRY, 2015, 470 : 7 - 13
  • [3] A mild synthesis of N-functionalised bromomaleimides, thiomaleimides and bromopyridazinediones
    Castaneda, Lourdes
    Wright, Zoe V. F.
    Marculescu, Cristina
    Tran, Trang M.
    Chudasama, Vijay
    Maruani, Antoine
    Hull, Elizabeth A.
    Nunes, Joao P. M.
    Fitzmaurice, Richard J.
    Smith, Mark E. B.
    Jones, Lyn H.
    Caddick, Stephen
    Baker, James R.
    [J]. TETRAHEDRON LETTERS, 2013, 54 (27) : 3493 - 3495
  • [4] Fluorescent Nanosensors Based on Fluorescence Resonance Energy Transfer (FRET)
    Chen, Gengwen
    Song, Fengling
    Xiong, Xiaoqing
    Peng, Xiaojun
    [J]. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 2013, 52 (33) : 11228 - 11245
  • [5] Virus-templated FRET platform for the rational design of ratiometric fluorescent nanosensors
    Chen, Limin
    Wu, Yehong
    Lin, Yuan
    Wang, Qian
    [J]. CHEMICAL COMMUNICATIONS, 2015, 51 (50) : 10190 - 10193
  • [6] Fluorescent Functionalization across Quaternary Structure in a Virus like Particle
    Chen, Zhuo
    Boyd, Stefanie D.
    Calvo, Jenifer S.
    Murray, Kyle W.
    Mejia, Galo L.
    Benjamin, Candace E.
    Welch, Raymond P.
    Winkler, Duane D.
    Meloni, Gabriele
    D'Arcy, Sheena
    Gassensmith, Jeremiah J.
    [J]. BIOCONJUGATE CHEMISTRY, 2017, 28 (09) : 2277 - 2283
  • [7] Dual Functionalized Bacteriophage Qβ as a Photocaged Drug Carrier
    Chen, Zhuo
    Li, Na
    Chen, Luxi
    Lee, Jiyong
    Gassensmith, Jeremiah J.
    [J]. SMALL, 2016, 12 (33) : 4563 - 4571
  • [8] Viral chemistry: the chemical functionalization of viral architectures to create new technology
    Chen, Zhuo
    Li, Na
    Li, Shaobo
    Dharmarwardana, Madushani
    Schlimme, Anna
    Gassensmith, Jeremiah J.
    [J]. WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY, 2016, 8 (04) : 512 - 534
  • [9] Tobacco Mosaic Virus Delivery of Phenanthriplatin for Cancer therapy
    Czapar, Anna E.
    Zheng, Yao-Rong
    Riddell, Imogen A.
    Shukla, Sourabh
    Awuah, Samuel G.
    Lippard, Stephen J.
    Steinmetz, Nicole F.
    [J]. ACS NANO, 2016, 10 (04) : 4119 - 4126
  • [10] Nitroxyl Modified Tobacco Mosaic Virus as a Metal-Free High-Relaxivity MRI and EPR Active Superoxide Sensor
    Dharmarwardana, Madushani
    Martins, Andre F.
    Chen, Zhuo
    Palacios, Philip M.
    Nowak, Chance M.
    Welch, Raymond P.
    Li, Shaobo
    Luzuriaga, Michael A.
    Bleris, Leonidas
    Pierce, Brad S.
    Sherry, A. Dean
    Gassensmith, Jeremiah J.
    [J]. MOLECULAR PHARMACEUTICS, 2018, 15 (08) : 2973 - 2983
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