The polysaccharide fraction of Propionibacterium acnes modulates the development of experimental focal segmental glomerulosclerosis

被引：9

作者：

Reis, Vanessa Oliveira ^{[1
]}

Silva, Janice Costa ^{[1
]}

Souza, Gabriela Trindade ^{[3
]}

Semedo, Patricia ^{[1
]}

Buscariollo, Bruna ^{[1
]}

Pereira, Rafael Luiz ^{[1
]}

Cenedeze, Marcos Antonio ^{[1
]}

Pacheco-Silva, Alvaro ^{[1
,2
]}

Longo-Maugeri, Ieda M. ^{[3
]}

Saraiva Camara, Niels Olsen ^{[1
,4
]}

Keller, Alexandre Castro ^{[1
,3
]}

机构：

[1] Univ Fed Sao Paulo, Dept Med, Sao Paulo, Brazil

[2] Inst Israelita Ensino & Pesquisa Albert Einstein, Unidade Transplante Renal, Sao Paulo, Brazil

[3] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil

[4] Univ Sao Paulo, Dept Imunol, Sao Paulo, Brazil

来源：

IMMUNOBIOLOGY | 2012年 / 217卷 / 09期

基金：

巴西圣保罗研究基金会;

关键词：

Adriamycin nephropathy; FSGS; Glomerulosclerosis; Immunomodulation; Propionibacterium acnes; PURIFIED SOLUBLE POLYSACCHARIDE; MURINE ADRIAMYCIN NEPHROPATHY; TO-MESENCHYMAL TRANSITION; TGF-BETA; DOXORUBICIN NEPHROPATHY; TRANSCRIPTION FACTOR; DEVELOPING GLOMERULI; PROTEINURIC STATES; RENAL INFLAMMATION; NEPHROTIC SYNDROME;

D O I：

10.1016/j.imbio.2011.12.003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The pathogenesis of focal segmental glomerulosclerosis (FSGS) appears to be associated with type-2 cytokines and podocyte dysfunction. In this study, we tested the hypothesis that immunization with the polysaccharide fraction of Propionibacterium acnes (PS), a pro-Th1 agonist, may subvert the type-2 profile and protect podocytes from adriamycin-induced glomerulosclerosis. Adriamycin injection resulted in albuminuria and increased serum creatinine in association with loss of glomerular podocin and podoplanin expression, which is consistent with podocyte dysfunction. Renal tissue analysis revealed the expression of transcripts for GATA3 and fibrogenic-related proteins, such as TGF-beta, tissue inhibitor of metalloproteinase-1 (TIMP-1) and metalloproteinase 9 (MMP9). In association with the expression of fibrogenic transcripts, we observed peri-glomerular expression of a-smooth muscle actin (alpha-SMA), indicating epithelial-to-mesenchymal transition, and increased expression of proliferating cell nuclear antigen (PCNA) in tubular cells, suggesting intense proliferative activity. Previous immunization with PS inhibited albuminuria and serum creatinine in association with the preservation of podocyte proteins and inhibition of fibrogenic transcripts and the expression of alpha-SMA and PCNA proteins. Tissue analysis also revealed that PS treatment induced expression of mRNA for GD3 synthase, which is a glycosiltransferase related to the synthesis of GD3, a ganglioside associated with podocyte physiology. In addition, PS treatment inhibited the influx of inflammatory CD8(pos) and CD11b(pos) cells to kidney tissue. Finally, PS treatment on day 4 post-ADM, a period when proteinuria was already established, was able to improve renal function. Thus, we demonstrate that the PS fraction of P. acnes can inhibit FSGS pathogenesis, suggesting that immunomodulation can represent an alternative approach for disease management. (C) 2011 Elsevier GmbH. All rights reserved.

引用

页码：831 / 841

页数：11

共 34 条

[1]

Barisoni L, 2009, ARCH PATHOL LAB MED, V133, P201, DOI 10.1043/1543-2165-133.2.201

[2] The Protective Role of Smad7 in Diabetic Kidney Disease: Mechanism and Therapeutic Potential [J].