A thermostable azo-linker for reversible photoregulation of DNA replication

被引：25
作者：
Wang, Qi
Yi, Long
Liu, Liangliang
Zhou, Chuanheng
Xi, Zhen [1 ]
机构：
[1] Nankai Univ, State Key Lab Elementoorgan Chem, Tianjin 300071, Peoples R China
来源：
TETRAHEDRON LETTERS | 2008年 / 49卷 / 34期
基金：
中国国家自然科学基金;
关键词：
DNA replication; reversible photoregulation; thermostable azo-linker; hairpin DNA;
D O I：
10.1016/j.tetlet.2008.06.027
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Small molecules such as azobenzenes, one of the best reversible photo-switches, can be covalently incorporated into DNA to regulate its structures and functions with irradiation of the specific wavelengths. Using this strategy, a thermostable azobenzene linker was employed to construct modified oligodeoxynucleotides, and we successfully achieve reversible photoregulation of DNA replication in vitro with short irradiation time. Five minutes UV irradiation for regulating trans -> cis transformation can minimize DNA damage, still ensure the polymerase reaction of cis-form. Formation of DNA hairpin structure can also be controlled by photoregulation using this linker. (c) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码：5087 / 5089
页数：3
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