Secretion of Escherichia coli DsbA and DsbC proteins from Brevibacillus choshinensis:: stimulation of human epidermal growth factor production

被引：6

作者：

Tanaka, R

Hashimoto, M

Ishibashi, M

Tokunaga, H

Taura, S

Mizukami, M

Miyauchi, A

Tokunaga, M

机构：

[1] Kagoshima Univ, Fac Agr, Lab Appl & Mol Microbiol, Kagoshima 8900065, Japan

[2] Kagoshima Univ, Ctr Gene Res, Kagoshima 8900065, Japan

[3] Higeta Shoyu Co, Dept Res & Dev, Chiba 2888680, Japan

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 22期

关键词：

Brevibacillus choshinensis; Dsb; epidermal growth factor; insulin; secretion;

D O I：

10.1023/A:1012798406359

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

DsbA and DsbC, members of the thioredoxin super-family of redox proteins, which are expressed in the periplasmic space of Escherichia coli, were cloned into and successfully secreted from Brevibacillus choshinensis at similar to 100 mug ml(-1). Both proteins were active in exchanging disulfide bonds of bovine insulin in vitro. Furthermore, DsbA secreted by B. choshinensis promoted the conversion of non-native human epidermal growth factor to the native form.

引用

页码：1853 / 1857

页数：5

共 15 条

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