Label free fluorescence turn-on detection of polynucleotide kinase activity with a perylene probe

被引：38

作者：

Jiao, Huping ^{[1
,2
]}

Wang, Bin ^{[1
,2
]}

Chen, Jian ^{[1
,2
]}

Liao, Dongli ^{[1
,2
]}

Yu, Cong ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 63期

基金：

中国国家自然科学基金;

关键词：

EXONUCLEASE;

D O I：

10.1039/c2cc32644b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A single stranded oligonucleotide could induce aggregation of a perylene probe, the probe's monomer fluorescence was efficiently quenched. However, when the oligonucleotide was 5'-phosphorylated by polynucleotide kinase, it could be very efficiently degraded by lambda exonuclease, probe monomers were released, and a turn on fluorescence signal was detected.

引用

收藏

页码：7862 / 7864

页数：3

相关论文

共 20 条

[1] BACTERIOPHAGE-T4 ANTICODON NUCLEASE, POLYNUCLEOTIDE KINASE AND RNA LIGASE REPROCESS THE HOST LYSINE TRANSFER-RNA [J].

AMITSUR, M ;

LEVITZ, R ;

KAUFMANN, G .

EMBO JOURNAL, 1987, 6 (08) :2499-2503

[2]

Busheri F. K., 1998, NUCLEIC ACIDS RES, V26, P4395

[3] Detection of enzymatic activity by PARACEST MRI:: A general approach to target a large variety of enzymes [J].

Chauvin, Thomas ;

Durand, Philippe ;

Bernier, Michele ;

Meudal, Herve ;

Doan, Bich-Thuy ;

Noury, Fanny ;

Badet, Bernard ;

Beloeil, Jean-Claude ;

Toth, Eva .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2008, 47 (23) :4370-4372

[4] Fluorometric detection of enzyme activity with synthetic supramolecular pores [J].

Das, G ;

Talukdar, P ;

Matile, S .

SCIENCE, 2002, 298 (5598) :1600-1602

[5] CONTROL OF EUKARYOTIC MESSENGER-RNA SYNTHESIS BY SEQUENCE-SPECIFIC DNA-BINDING PROTEINS [J].

DYNAN, WS ;

TJIAN, R .

NATURE, 1985, 316 (6031) :774-778

[6] Functional imaging of proteases: recent advances in the design and application of substrate-based and activity-based probes [J].

Edgington, Laura E. ;

Verdoes, Martijn ;

Bogyo, Matthew .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2011, 15 (06) :798-805

[7] Genome maintenance mechanisms for preventing cancer [J].

Hoeijmakers, JHJ .

NATURE, 2001, 411 (6835) :366-374

[8] Xrcc4 physically links DNA end processing by polynucleotide kinase to DNA ligation by DNA ligase IV [J].

Koch, CA ;

Agyei, R ;

Galicia, S ;

Metalnikov, P ;

O'Donnell, P ;

Starostine, A ;

Weinfeld, M ;

Durocher, D .

EMBO JOURNAL, 2004, 23 (19) :3874-3885

[9] Sensitive and Rapid Screening of T4 Polynucleotide Kinase Activity and Inhibition Based on Coupled Exonuclease Reaction and Graphene Oxide Platform [J].

Lin, Lei ;

Liu, Yang ;

Zhao, Xin ;

Li, Jinghong .

ANALYTICAL CHEMISTRY, 2011, 83 (22) :8396-8402

[10] Highly sensitive detection of DNA phosphorylation by counting single nanoparticles [J].

Ma, Changbei ;

Yeung, Edward S. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 397 (06) :2279-2284