Effect of Copper on the Expression of IGF-1 from Chondrocytes in Newborn Piglets In Vitro

被引：6
|
作者：
Wang, Jianguo [1 ]
Zhu, Xiaoyan [1 ]
Xie, Guanghong [1 ]
Zhang, Yonghong [1 ]
Liu, Zhaoxi [1 ]
Zhang, Liang [1 ]
Zhao, Chenxu [1 ]
Yang, Wentao [1 ]
Shi, Xiaoxia [1 ]
Wang, Zhe [1 ]
Liu, Guowen [1 ]
机构：
[1] Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130062, Peoples R China
来源：
BIOLOGICAL TRACE ELEMENT RESEARCH | 2012年 / 148卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Copper; Chondrocyte; IGF-1; mRNA; Gene expression; GROWTH-FACTOR-I; ARTICULAR CHONDROCYTES; GENE-EXPRESSION; INSULIN; CARTILAGE; HORMONE; BONE; OSTEOARTHRITIS; PLATE;
D O I：
10.1007/s12011-012-9359-z
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The experiment was performed to evaluate the influence of copper supplementation on insulin-like growth factor-1 (IGF-1) mRNA expression in chondrocytes of newborn pigs. Chondrocytes were isolated and cultured in media containing 15% fetal calf serum supplemented with 0, 15.6, 31.2, and 62.5 mu mol/L copper in 90-mm culture plate. After 0, 12, 24, and 48 h, total RNA was isolated from chondrocytes. Then, IGF-1 mRNA expression was determined by semiquantitative RT-PCR. The results showed that the expression of IGF-1 mRNA, adjusted for beta-actin expression, was increased in the culture media added to 15.6, 31.2, and 62.5 mu mol/L copper, respectively. In the present experiment, the optimal copper concentration and optimal culture time for the expression of IGF-1 mRNA were 31.2 mu mol/L and 48 h, respectively.
引用
收藏
页码：178 / 181
页数：4
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