A trifunctional enzyme with glutathione S-transferase, glutathione peroxidase and superoxide dismutase activity

被引：57

作者：

Yan, Fei ^{[1
]}

Yang, Wen-kui ^{[1
]}

Li, Xin-yang ^{[1
]}

Lin, Ting-ting ^{[1
]}

Lun, Yan-ni ^{[1
]}

Lin, Feng ^{[1
]}

Lv, Shao-wu ^{[1
]}

Yan, Gang-lin ^{[1
]}

Liu, Jun-qiu ^{[2
]}

Shen, Jia-cong ^{[2
]}

Mu, Ying ^{[1
,3
]}

Luo, Gui-min ^{[1
]}

机构：

[1] Jilin Univ, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130023, Peoples R China

[2] Jilin Univ, Minist Educ, Key Lab Supramol Struct & Mat, Changchun 130023, Peoples R China

[3] Zhejiang Univ, Res Ctr Analyt Instrumentat, Hangzhou 310058, Peoples R China

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2008年 / 1780卷 / 06期

基金：

中国国家自然科学基金;

关键词：

reactive oxygen species; trifunctional enzyme; glutathione S-transferase; superoxide dismutase; glutathione peroxidase; enzyme mimic; fusion protein; metal incorporation;

D O I：

10.1016/j.bbagen.2008.03.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione S-transferase (GST) and glutathione reductase (GR) play crucial roles in balancing the production and decomposition of reactive oxygen species (ROS) in living organisms. These enzymes act cooperatively and synergistically to scavenge ROS, as not one of them can singlehandedly clear all forms of ROS. In order to imitate the synergy of the enzymes, we designed and generated a recombinant protein, which comprises of a Schistosoma japonicum GST (SjGST) and a bifunctional 35-mer peptide with SOD and GPX activities. The engineered protein demonstrated SOD, GPX and GST activities simultaneously. This trifunctional enzyme with SOD, GPX and GST activities is expected to be the best ROS scavenger. (c) 2008 Elsevier B.V. All rights reserved,

引用

页码：869 / 872

页数：4

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